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Anti-inflammatory properties of Holothuria atra extract on lipopolysaccharide induced inflammation of White blood cells

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Last updated: 04 Jan 2025

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Abstract

Holothuria atra is a marine invertebrate found in the tropical indo-pacific region. It contains potent bioactive compounds, which include lectin, steroidal saponins, and triterpene glycoside. The anti-inflammatory and antioxidant characteristics of Holothuria atra extract have been investigated in-vitro in this study. Ethyl acetate extract of H. atra was prepared by maceration methods, and then the phytochemical compositions were measured colorimetric and by using gas chromatography-mass spectrometry (GC-MS) Analysis. Hemolytic activity and anti-inflammatory properties of the extract were measured through cell culture protocol. The obtained results showed that the extract has a high phenol content (121.92±0.14 mg/g), and GC-MS analysis revealed that it contains, Oleic acid, 9-octadecenoic acid, methyl ester, and Octadecanoic acid.
Moreover, antioxidant properties were determined by assessing 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing power, hydroxyl radical scavenging, and American Board of Thoracic Surgery (ABTS) scavenging activity. Furthermore, it is concluded that H. atra inhibited the stimulated Lipopolysaccharide (LPS) - Inducible nitric oxide synthase (iNOS) gene expression and protein expression by 6.17±0.01-fold change and Cyclooxygenase-2 (COX-2) gene expression by 4.70±0.05-fold change. The crude extract of H. atra could be considered a good anti-inflammatory agent.

DOI

10.21608/djvs.2022.157375.1090

Keywords

Sea cucumbers, Antioxidant activity, DPPH, MTT assay, COX-2, iNOS

Authors

First Name

Safaa

Last Name

Awad

MiddleName

Nageb

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

safaanageb541@gmail.com

City

shubrakhit

Orcid

29408011802662

First Name

Mohammed

Last Name

Abd El Hafez

MiddleName

Said

Affiliation

National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt, Center of Excellence for Drug Preclinical Studies (CE DPS), Pharmaceutical and Fermentation Industries Development Centre (PFIDC), City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab, Egypt

Email

mohamedsaid80@yahoo.com

City

Alexandria

Orcid

0000-0002-7223-2340

First Name

Miral

Last Name

Abd Elwahab

MiddleName

Gamal

Affiliation

Center of Excellence for Drug Preclinical Studies (CE DPS), Pharmaceutical and Fermentation Industries Development Centre (PFIDC), City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab, Egypt

Email

dr.miral2013@yahoo.com

City

Alexandria

Orcid

0000-0002-4108-7136

First Name

Rehab

Last Name

Mady

MiddleName

-

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

rehabmady1976@gmail.com

City

-

Orcid

-

First Name

Gaber

Last Name

Batiha

MiddleName

El-Saber

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

gaberbatiha@gmail.com

City

-

Orcid

0000-0002-7817-425X

First Name

Hazem

Last Name

Shaheen

MiddleName

M

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

dr_hazemshaheen3010@gmail.com

City

Damanhour

Orcid

0000-0001-5931-0466

First Name

Doaa

Last Name

Ghareeb

MiddleName

Ahmed

Affiliation

Center of Excellence for Drug Preclinical Studies (CE DPS), Pharmaceutical and Fermentation Industries Development Centre (PFIDC), City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab, Egypt, Bio screening and Preclinical Trial Lab, Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt

Email

d.ghareeb@alexu.edu.eg

City

Alexandria

Orcid

0000-0003-3327-4377

Volume

9

Article Issue

2

Related Issue

39844

Issue Date

2023-02-01

Receive Date

2022-08-20

Publish Date

2023-03-07

Page Start

9

Page End

16

Print ISSN

2636-3003

Online ISSN

2636-3011

Article File

DJVS_Volume 9_Issue 2_Pages 9-16.pdf

PDF . 482.0kB

Link

https://djvs.journals.ekb.eg/article_289200.html

Detail API

https://djvs.journals.ekb.eg/service?article_code=289200

Order

289,200

Type

Original Article

Type Code

1,001

Publication Type

Journal

Publication Title

Damanhour Journal of Veterinary Sciences

Publication Link

https://djvs.journals.ekb.eg/

MainTitle

Anti-inflammatory properties of Holothuria atra extract on lipopolysaccharide induced inflammation of White blood cells

Details

Type

Article

Created At

24 Dec 2024