The present study was conducted to evaluate the effects of different dietary zinc sources on carcass traits, mortality, cell-mediated immune response, physiological traits, and economic evaluation of broiler chickens. A total of 156 newly hatched chicks (Ross 308) were randomly distributed into four treatments. The dietary treatment received the basal diet without zinc supplementation (T1) was considered as the control group, the T2 supplemented with 40 mg Zn /kg diet inorganic zinc oxide, T3 supplemented with 40 mg Zn/kg diet organic zinc-lysine, and T4 supplemented with 40 mg /kg diet with a prepared nano zinc oxide (NZnO). The data stated that nano zinc oxide (T4) and zinc lysine (T3) significantly recorded the highest carcass weight and breast muscle weight (P <0.05) compared to chicks in T1 and T2.  Nano zinc oxide (T4) significantly (P<0.05) achieved the highest thigh with drumstick weight compared to T2. None of the different organ indices percentages (internal organs and lymphoid immune organs) were significantly affected (P > 0.05) by different Zn sources. The mortality % was not significantly affected by dietary treatments. Non-significant (P<0.05) differences were observed in the serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), creatinine, and uric acid levels among treatments. Using NZnO (T4) significantly gave a better selling price compared to T1, T2, and T3. It could be concluded that nano zinc oxide, 40 mg Zn/kg diet, has the highest economical return with no adverse effect physiological status of broilers and can be safely included as a good feed additive in broiler diets.