This study included Salicornia fertcusa plants collected from 7 locations in Lake Manzala, Port Said, Egypt during the winter season of 2021. The fixed oil was extracted from air dried seeds with hexane using Soxchelt apparatus. The major fatty acids of the fixed oil were studied qualitatively and quantitatively with GC-MS after methylation. The results showed that: 1) Fourteen fatty acids were identified in the fixed oil, which accounted for ca 98% of the total fatty acids. 2) Palmitic acid was identified as the major fatty acid in the Salicornia fertcusa seeds of all collected plants from different stands and ranged from 19.58 to 30.62% of the total fatty acids of the fixed oil. 3) Statistical analyses indicated that the fixed oil percentages and its main fatty acids were changed according to the soil content of Ca²⁺, Mg, K⁺, Na⁺, and Cl^-, soil electrical conductivity values, and   its physical characteristics.