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360840

Molecular Epidemiology of Foot-and-Mouth Disease Virus in Tanzania during 2020 to 2021 Outbreaks

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Veterinary microbiology and pathobiology (Veterinary Bacteriology & my…irology, immunology, parasitology, pathology, and molecular biology).

Abstract

Background: Food and mouth disease (FMD) is an endemic disease of cattle and other cloven hoofed animals. The objective of this study was to investigate the genetic characteristics and evolutionary relationships for the 2020 to 2021 field circulating Food and mouth disease virus (FMDV) obtained from reported outbreaks in different parts of Tanzania.
Methods: The epithelial tissues were collected from lesions (oral, nasal, interdigital) of FMD suspect cases, stored and shipped to the laboratory for analysis. In the laboratory, the samples were prepared for nucleic acid extraction, FMDV detection, typing, sequencing, and phylogeny analysis. The construction of the phylogenetic trees was done by aligning current field strains nucleotide sequences with those from past studies stored in GenBank database.   
Results: The study identified three FMDV serotypes (A, O and, SAT1) to be circulating in the field as Africa Topotype G-I lineage, EA-2 Topotype, and Topotype I (NWZ) respectively. The identified field strains showed diverse scores of shared identity among current and past study strains. The generated nucleotide sequences from this study types O and SAT1 field strains were analysed categorically, and showed shared percent identities of 92.0-100.0% and 96.9-98.8% respectively.
Conclusion: The sequencing and analysis of the VP1 coding region enhance FMDV knowledge on the genetic and evolutionary relationships existing among field strains, and commend for improved future strategies for effective national, regional and global FMD control measures.

DOI

10.21608/jvmr.2024.276435.1099

Keywords

FMDV, Serotype A, Serotype O, Serotype SAT1, Tanzania

Authors

First Name

Mkama

Last Name

Mashinagu

MiddleName

Mathias

Affiliation

Department of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro 3019, Tanzania. , SACIDS Foundation for One Health, Africa Centre of Excellence for Infectious Diseases of Humans and Animals, Morogoro 3297, Tanzania., Tanzania Veterinary Laboratory Agency, Centre for Infectious Diseases and Biotechnology, Dar es salaam 9254, Tanzania.

Email

mathias.mkama@sacids.org

City

-

Orcid

-

First Name

Philemon

Last Name

Wambura

MiddleName

-

Affiliation

Department of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro 3019, Tanzania.

Email

pwambura@sua.ac.tz

City

Morogoro

Orcid

-

First Name

Sharadhuli

Last Name

Kimera

MiddleName

-

Affiliation

Department of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro 3019, Tanzania., Department of Veterinary Medicine and public Health, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro 3021, Tanzania.

Email

sharadhuli.kimera@sacids.org

City

Morogoro

Orcid

-

First Name

Donald

Last Name

King

MiddleName

-

Affiliation

FAO World Reference Laboratory for FMD, The Pirbright Institute, Ash Road, Pirbright, Woking, GU24 ONF, United Kingdom.

Email

donald.king@pirbright.ac.uk

City

London

Orcid

-

First Name

David

Last Name

Paton

MiddleName

-

Affiliation

FAO World Reference Laboratory for FMD, The Pirbright Institute, Ash Road, Pirbright, Woking, GU24 ONF, United Kingdom.

Email

dajapaton@gmail.com

City

London

Orcid

-

First Name

Francois

Last Name

Maree

MiddleName

-

Affiliation

Clinomics, Uitzich Road, Bainsvlei, Bloemfontein, Free State 9301, South Africa.

Email

francois.maree@clinglobal.com

City

Free State

Orcid

-

First Name

Herbertha

Last Name

Mpete

MiddleName

-

Affiliation

Department of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro 3019, Tanzania.

Email

herberthampete@gmail.com

City

Morogoro

Orcid

-

First Name

Sengiyumva

Last Name

Kandusi

MiddleName

-

Affiliation

Department of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro 3019, Tanzania.

Email

sengiyumvakandusi@gmail.com

City

Morogoro

Orcid

-

First Name

Mark

Last Name

Rweyemamu

MiddleName

M.

Affiliation

Department of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro 3019, Tanzania., SACIDS Foundation for One Health, Africa Centre of Excellence for Infectious Diseases of Humans and Animals, Morogoro 3297, Tanzania.

Email

mark.rweyemamu@sacids.org

City

-

Orcid

-

First Name

Christopher

Last Name

Kasanga

MiddleName

-

Affiliation

Department of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro 3019, Tanzania., SACIDS Foundation for One Health, Africa Centre of Excellence for Infectious Diseases of Humans and Animals, Morogoro 3297, Tanzania.

Email

christopher.kasanga@sua.ac.tz

City

Morogoro

Orcid

-

Volume

31

Article Issue

1

Related Issue

48528

Issue Date

2024-06-01

Receive Date

2024-03-13

Publish Date

2024-06-01

Page Start

15

Page End

22

Print ISSN

2357-0512

Online ISSN

2357-0520

Article File

JVMR_Volume 31_Issue 1_Pages 15-22.pdf

PDF . 1.7MB

Link

https://jvmr.journals.ekb.eg/article_360840.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=360840

Order

360,840

Type

Original Article

Type Code

891

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

Molecular Epidemiology of Foot-and-Mouth Disease Virus in Tanzania during 2020 to 2021 Outbreaks

Details

Type

Article

Created At

24 Dec 2024