There is a great exertion to look for the alternative therapeutic methods that assist in controlling the antibacterial resistance of Salmonellae. The aim of this study was to evaluate the antibacterial effect of vitamin C (ascorbic acid) different formulations at different concentrations. Serotyping of the isolates revealed that 10 belonged to S. Enteritidis 1,9,12:g,m, 7 S. Virchow 6,7,14:r:1,2, and 3 S. Montevideo 6,7,14:g,m[p],s :[1,2,7]. The isolates were highly resistant (100%) to amoxicillin-clavulanic acid, streptomycin, erythromycin, clindamycin, and doxycycline, and 90% of the isolates were susceptible to amikacin. PCR revealed that 10 S. Enteriditis isolates harbored 3 virulence-associated genes invA, sefA, and fimH, while 7 isolates harbored stn gene. Also, 10 S. Enteriditis isolates harbored sul1 and bla_TEM genes. Biofilm formation was 100%, 50%, and 42.8% in S. Montevideo, S. Enteritidis, and S. Virchow respectively. Vit C (formulation 1) completely inhibited the growth of all isolates at concentrations 0.3%, 0.5% and 1%. Formulation 2 inhibited the growth of the tested isolates at concentration 0.3%. While, concentration 0.1% inhibited only the growth of S. Virchow. Formulation 3 inhibited the growth of S. Enteritidis and S. Virchow at concentrations 0.3 and 0.5%. Formulation 4 inhibited the growth S. Enteritidis, S. Virchow and S. Montevideo at concentration 1%. Formulation 5 inhibited the growth of S. Enteritidis and S. Virchow at concentrations 0.1%, 0.5% and 1%. Formulations 4 and 1 inhibited effectively the biofilm-forming ability of S. Enteritidis (80% and 100%) and S. Virchow (60% and 66.6%).