There is a great exertion to look for alternative therapeutic methods that assist in controlling the antibacterial resistance of Salmonellae. This study aimed to evaluate the antibacterial effect of vitamin C (ascorbic acid) in different formulations at different concentrations. Serotyping of the isolates revealed that 10 belonged to S. Enteritidis 1,9,12:g,m, 7 S. Virchow 6,7,14:r:1,2, and 3 S. Montevideo 6,7,14:g,m[p],s :[1,2,7]. The isolates were highly resistant (100%) to amoxicillinclavulanic acid, streptomycin, erythromycin, clindamycin, and doxycycline, and 90% of the isolates were susceptible to amikacin. PCR revealed that 10 S. Enteritidis isolates harbored 3 virulence-associated genes invA, sefA, and fimH, while 7 isolates harbored stn gene. Also, 10 S. Enteritidis isolates harbored sul1 and blaTEM genes. Biofilm formation was 100%, 50%, and
42.8% in S. Virchow, S. Enteritidis, and S. Montevideo, respectively. Vitamin C (formulation 1) completely inhibited the growth of all isolates at concentrations of 0.3%, 0.5%, and 1%. Formulation 2 inhibited the growth of the tested isolates at a concentration of 0.3%. While a concentration of 0.1% inhibited only the growth of S. Virchow. Formulation 3 inhibited the growth of S. Enteritidis and S. Virchow at concentrations of 0.3 and 0.5%. Formulation 4 inhibited the growth of S. Enteritidis, S. Virchow, and S. Montevideo at a concentration of 1%. Formulation 5 inhibited the growth of S. Enteritidis and S. Virchow at concentrations 0.1%, 0.5%, and 1%. Formulations 4 and 1 inhibited effectively the biofilm-forming ability of S. Enteritidis (80% and 100%) and S. Virchow (60% and 66.6%).