This study was conducted to the isolation and identification of pathogenic bacteria from different chicken organs in Al-sharkia government using biochemical and standard culture procedures.. A total of 100 chicken organs samples ( Breast , Liver , Drumstick , Wings , Skin , Intestine , Pins, Giblets , Heart and Legs ) from local chicken were collected during a period from November 2021 and May 2022, the bacteria were diagnosed according to morphological, cultural and biochemical characteristics. Bacteriological examination of the samples showed both Gram-positive and Gram-negative bacteria Breast (n=22) , Drumstick (n=18) , Liver (n=11) , Wings(n=12) , Skin (n=4) , Intestine (n=12) , Pins (n=8) , Giblets (n=12) , Heart (n=8) , Legs (n=5) with isolation rates of 19.6% , 16.1% , 9.82% , 10.7% , 3.6% , 10.7% , 7.14% , 10.7% , 7.14% , 4.5 % as well as to examine the phenotypic and genetic relationships between bacterial isolates from different chicken organs that were resistant to critically important antibiotics. Bacillus species that have been proven were tested for their biochemical character.