In April and May 2023, five skin nodule samples were collected from cattle suspected to have lumpy skin disease (LSD) in three governorates in Egypt: Dakahlia, El-Menia, and El-Fayoum. Lumpy skin disease virus (LSDV) was detected quickly and specifically using Taq-Man real-time PCR. All samples proving positive for the virus. Following that, one positive sample from El-Fayoum governorate was isolated on Madin-Darby bovine kidney (MDBK) tissue culture for three passages, and characteristic LSDV cytopathic effects (CPE) were observed. To molecularly characterize and differentiate the strain, the isolated sample was subjected to sequencing and phylogenetic analysis, with a focus on the partial sequence of ORF 103. The results showed that the isolated virus belonged to the field strain group of LSDV, which was separate from vaccinal strains, demonstrating that ORF 103 sequencing data can be used to differentiate between field wild strains and vaccinal strains, as well as other Capripoxviruses (CaPVs).