The E. coli biofilm is made up of a bacterial colony that is immersed in an extracellular polymeric substance (EPS) matrix that shields the microorganisms from harmful environmental factors and causes infection. The development of antibiotic resistance exacerbates the diversity in structural elements of biofilm, making it more difficult to eradicate. The present work review focuses on a variety of E. coli issues. So, the study aim is biofilm production evaluation in E. coli isolated from the broiler water system.In this study, one hundred swabs were collected from drinking water pipes and drinkers from different broiler farms in Giza and Dakahlia governorates in Egypt. The swabs were examined for the presence of E. coli and their ability for biofilm production. Fifty swabs were positive for E. coli after biochemical and serological identification of isolates. Microtiter-plate test for determination of biofilm production showed that forty isolates produce biofilm with different degrees, classified 13 (26%) strong biofilms, 17(34%) intermediate biofilms and 10(20%) weak biofilms,10 isolates out of 50 (20%) exhibited no biofilm. The PCR detection of the adrA gene in E. coli isolates originating from broiler environments was performed, specifically focusing on the thirteen strong biofilm-forming isolates.