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Detection of Salmonella Enteritidis (sefA) gene, isolated from internal organs of broiler Chickens

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Microbiology

Abstract

Salmonella is one of genus under the family Enterobacteriaceae and recorded as an important zoonotic pathogen. Salmonella infection ( salmonellosis ) is a serious problem to the chicken farms in different areas because the broiler represented its important host. S. Enteritidis strain affected mainly internal organs of the chicken, resulting in elevation of the morbidity and mortality rates, consequently a high economic loses in the broiler production. The aim of this work was the detection of S. Enteritidis(sefA) gene, isolated from internal organs of healthy slaughtered broiler Chickens. The total samples 129, were determined as follow: (Liver, kidneys, small intestine, gizzard and heart blood) are ( 20, 19, 37, 30, 23) respectively. The samples were collected under microbiological examination. The incidence of Salmonella Species was tabulated as flow: (20%, 10.52%, 37.83%, 6.66% and 8.69%) from liver, kidneys, small intestine, gizzard and heart blood respectively. Only 8 isolates from 24 isolated strains were under go serological tests, recording one serotype of Salmonella Enteritidis. The high prevalence occurred in small intestine and liver of apparently healthy broilers, and the lower prevalence occurred in heart blood, kidneys and gizzard of internal organ of broilers. The data resulted from antimicrobial sensitivity test application; high resistant rate to vancomycin (64.6%); gentamicin (64.6%); tetracycline (92.8%); chloramphenicol, (85.7%); ciprofloxacin (35.7%); levofloxacin, (64.28%); amoxicillin+clavulanic, (100%); streptomycin, (92.85); trimethoprim+sulfamethoxaole (85.7%). while the sensitivity to florfenicol was 100%. Confirmatory PCR technique for detection of (sefA) gene amplification at ( 310 bp) in 8 isolated strain.

DOI

10.21608/bvmj.2023.239271.1731

Keywords

Salmonella Enteritidis, Broilers, sefA

Authors

First Name

Abdulkareem

Last Name

Hamad

MiddleName

Shihab

Affiliation

1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, Kalyobiya, Egypt 2Ministry of Agriculture, Veterinary Department, Iraq

Email

altammeer@gmail.com

City

veterinary medicine faculty

Orcid

-

First Name

Fatma

Last Name

El-hofy

MiddleName

I.

Affiliation

Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, Kalyobiya, Egypt

Email

fatma.abdlallah@fvtm.bu.edu.eg

City

-

Orcid

-

First Name

Ramzy

Last Name

Hamouda

MiddleName

-

Affiliation

Animal Reproductive Research Institute (ARRI), Haram, Giza, Egypt.

Email

ramzyhamouda71@gmail.com

City

-

Orcid

-

First Name

Ashraf

Last Name

Awad

MiddleName

-

Affiliation

Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, Kalyobiya, Egypt

Email

ashraf.awad@fvtm.bu.edu.eg

City

Toukh

Orcid

0000-0002-1221-3340

Volume

45

Article Issue

2

Related Issue

46335

Issue Date

2023-12-01

Receive Date

2023-09-28

Publish Date

2023-12-01

Page Start

117

Page End

120

Print ISSN

1110-6581

Online ISSN

2974-4806

Link

https://bvmj.journals.ekb.eg/article_343574.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=343574

Order

343,574

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Detection of Salmonella Enteritidis (sefA) gene, isolated from internal organs of broiler Chickens

Details

Type

Article

Created At

24 Dec 2024