Hepatitis B virus (HBV) is a blood-borne virus that poses chief public health threats and it is the frequent reason for cirrhosis all over the world. microRNA could be used as disease biomarkers. This investigation was carried out to investigate mRNA -125b, mRNA 489-3p and biochemical analysis as marker for chronic HBV. This work included 20 patients had chronic liver disease admitted to the Gastroenterology Hospital and Oncology Institute in Mansoura. After investigating the patients, 5 patients, from the 20 patients, were positive PCR chronic HBV. Serum samples were obtained from those 5 patients. For control, negative serum samples were collected at the same age range and sex. Liver function parameters and AFP were estimated. RNA was extracted from serum for gene expression of mRNA125b and mRNA 489-3p. The main results were; HBV group showed a significant increase in ALT, AST, TBIL, GGT, ALP while there was significant decrease in albumin levels. In HBV group AFP was significantly increased compared to control. HBV group revealed a significant increase (6.70 ± 0.18) in mRNA125b, with a significant decrease in mRNA489-3p (0.51 ± 0.03). On conclusion, mRNA125b and mRNA489-3p expressions along with biochemical analysis could be used as an accurate marker for HBV diagnosis.
Keywords: microRNA 125b , microRNA 489-3p, HBV.