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296314

In vitro maturation (IVM) of camel oocytes using different maturation media

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Theriogenology

Abstract

The aim of the present study is to evaluate the in vitro maturation rate of camel oocytes using different maturation media. Ovaries of 25 she-camel were collected during the breeding season (from December to April). Ovaries were transferred to the lab in a thermal flask containing warm saline solution within 2 hrs. after slaughtering. Oocytes were collected using 2 techniques; aspiration of graafian follicles and slicing of the ovaries. Depending on the morphological quality, the collected oocytes were selected and cultured on different types of maturation media for 30-40 hours at 38.5˚c, 5% CO2, and maximum humidity (95-96%). The obtained results revealed that the slicing technique produced a higher oocyte recovery rate compared to the aspiration technique. In addition, the maturation media composition mainly the FSH affects the in vitro maturation rate of camel oocytes. It could be concluded that the media modification used in oocytes maturation might be one of the factors that improve the rate of oocyte maturation.

DOI

10.21608/bvmj.2023.168049.1604

Keywords

Dromedary camel, maturation media, oocyte maturation, oocyte retrieval

Authors

First Name

Shimaa

Last Name

Gwaid

MiddleName

Adel

Affiliation

Theriogenology, Benha university, Benha, Egypt

Email

shimoadel251@gmail.com

City

Cairo

Orcid

-

First Name

Ahmed

Last Name

Reda

MiddleName

-

Affiliation

Theriogenology department, Faculty of Veterinary Medicine, Benha University

Email

drahmed_reda@yahoo.com

City

-

Orcid

-

First Name

Mohsen

Last Name

Aggag

MiddleName

-

Affiliation

Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Egypt.

Email

mohsan.aggag@fvtm.bu.edu.eg

City

Toukh

Orcid

-

First Name

Alaa

Last Name

Abdelghafar

MiddleName

-

Affiliation

Theriogenology department -benha university- faculty of veterinary medicine.

Email

abdel_ghafar@yahoo.com

City

El- Qalibua

Orcid

-

Volume

44

Article Issue

1

Related Issue

40949

Issue Date

2023-04-01

Receive Date

2022-10-17

Publish Date

2023-04-01

Page Start

30

Page End

33

Print ISSN

1110-6581

Online ISSN

2974-4806

Link

https://bvmj.journals.ekb.eg/article_296314.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=296314

Order

296,314

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

In vitro maturation (IVM) of camel oocytes using different maturation media

Details

Type

Article

Created At

24 Dec 2024