Present study on Egyptian Fruit Bat (Rousettus aegyptiacus) conduct on 60 apparently healthy bats (19 young, 36 adults and 5 neonate) and designed to investigate data base in this species, bat's morphometric measurements needed to determine all bat's external characters which help in identification of different species and different age or sex. morphometric parameters measured by ruler, show significant difference between different age and no effect of sex, overall mean of body weight 111.1±34.29 g, Forearm 8.74±0.85 cm, body length 14.26±1.58 cm and Wingspan 61.18±6.41 cm. Evaluation of hematological and biochemical parameters of animals are very important to diagnosis diseases and to determine a requirements of nutrition to them. hematological parameters measured by Hemocytometer show significant difference between different age in RBC, MCV,  Eosinophil  and no effect of sex, overall mean of  RBC 7.19±2.56 10¹²/l, WBC 6.87±3.96 10⁹/l, HB 13.99±1.96 g/dl and Platelets 199.3±118.9 10⁹/l. biochemical parameters measured by Spectrophotometer show significant difference between different age in triglyceride and between different sex in total calcium and cholesterol, overall mean of AST 219.5±191.2 IU/l, ALT 115.6±77.68 IU/l, Total calcium 1.85±0.40 mmol/l, Urea 3.98±3.48 mmol/l, Cholesterol 0.26±0.15 mmol/l, Glucose 5.37±1.70 mmol/l.