Kombucha tea (KT) is a traditional non-alcoholic beverage, which is produced from the fermentation of black or green tea with a combination of acetic acid bacteria (AAB) and yeast. In addition to its historical usage in traditional medicine for various ailments, recent studies have demonstrated its potential as anti-cancer and anti-viral agent. Therefore, the objective of this study was to investigate the impact of KT on inducing anti-cancer effects in HepG-2 cells and its potential to exhibit anti-viral activity using Vero cells. MTT assay was conducted to assess the cytotoxicity of the KT and choose the optimal concentration for the study. Three types of cells: Normal human liver cells, HepG-2 cells, and Vero cells treated with KT doses of 10, 25, 50, 100, and 200 mg/ml. The IC₅₀ concentration recorded was 54.87 mg/ ml for HEPG-2 tumor cell line and 73.8 mg/ ml for Vero cells. It was demonstrated that the KT had a strong cytotoxic effect on tumor cells as well as an antiproliferative function.  However, it exhibited no discernible growth inhibition on normal cell lines. Flow cytometry was employed to study the KT's impact on inducing apoptosis (cell death), by exposing the cells to the KT for different periods (24, 48, 72 hours). It was found that KT has the ability to kill cancer-infected cells compared to the control, in early and late apoptosis of 11.27% and 11.01% respectively. The upregulation of the Bax and P53 genes and the downregulation of the Bcl-2 genes were part of the apoptotic process brought on by KT. These results showed that KT had an antiproliferative effect on HepG-2 cells through apoptosis-mediated cytotoxicity and cell cycle blockage at the G2/M phase. Regarding KT antiviral activity, it had a variable activity against different virus models. The percentage of depletion rate compared to its original viral titer recording was 23.1 %, 9.3% and 8.3% for Hepatitis A virus (HAV), rift valley fever virus (RVFV) and Herpes simplex virus (HSV), respectively. These findings imply that KT might be a good natural product therapy for anti-virus and anti-cancer medicines.