Twent non-cycling buffalo-heifers were used. The heifers were treated by exogenous sex steroids synchro-Mate-B" plus equine chorionic gonadotrophins (eCG) with or without carboxylic atmoshore (Lasalocid) supplement for induction of
estrus. Blood samples were collected from all heifers before treatment and from ten positively responded heifers after treatment. Blood samples from ten responded heifers (before and after most of the metabolic profile parameters.
ment) in each group were subjected to study effect of hormonal induction of cyclicity on metabolic profile testing. The results revealed the hormonal treatment without Lasalocid supplement did not alter Hb concentration, pcv%, ALT activity, total protein, albumin ; nitrogen concentrations. While, the activities ‘plasma AST and ALP were significantly increased and CPK activity, glucose and cholesterol concentrations were increased. chro-Mate-B implants' plus eCG   administration ‘n Lasalocid supplemented cycling differs did not lead to significant alterations in most of metabolic profile testing except PCV% and glucose concentration as compared to non cycling bufflao-heifers supplemented with Lasalocid Concentrations of plasma macro- and micro elements (Ca, Mg, Na, K and lithium) after induction of estrus by sex steroids plus cCG were not significantly differed in heifers either supplemented or not with Lasalocid. Thus, we can conclude that usage of Synchro-Mate-B implant plus eCG to induce estrus in prepubertal buffalo-heifers did not alter most of the metabolic profile parameters.