The immune response against ILT vaccination taking, different routes (intraocular, spray, dipping —_al., 1983).
id drinking water) was evaluated. The criteria of evaluation depended on quantitative agar gel recipitation test (QAGPT), counter immunoelctrophoreasis (QCIE), lymphocyte transformation (LT) test and protection against challenge of vaccinated and control groups. The results indicated that no significant difference between different vaccinated group either by QAGPT nor QCIE while revealed significant difference between different vaccinated group on the following descending order intraocular, spray, drinkng water and dipping route. Results of challenge revelaed maximum protection (90%) in occular instillation and spray procedures (inspite of adverse post vaccination reaction was recorded in spray vaccination) followed by drinking water jute (80%) while dipping and non vaccinated drinking water groups gave 40% and 20% respectively.