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378349

EVALUOLATION OF EGG YOLK IMMUNOGLOBULINS (IgY) BY CHLOROFORM POLYETHYLENE GLYCOL TECHNIQUE AND ASSAYING OF ANTIBODIES AGAINST AVIAN INFECTIOUS BRONCHITIS

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

Bacteriology and bacterial diseases

Abstract

The present research study was conducted to evaluate the chicken egg yolk immunoglobulins (IgY) by Chloroform Polyethylene Glycol (CPEG) technique and measure antibody titres against Avian Infectious Bronchitis (AIB). For purpose 300 eggs of broiler breeder were occured from 10 different flocks (30 eggs from h), at least 3 to 4 weeks postvacciantion 
against Avian Infectious Bronchitis (AJB). The of 3 eggs was pooled and subjected to the of IgY by CPEG technique. The
centration of purified IgY was quantified by spechtrophotometer and antibody titres against AIB measured through haemagglutination (H I) test. concentration of IgY in different flocks from 2.65 to 3.70 g/dl with overall mean 24 g/dl. The geomean antibody titres (GMT) AIB ranged from 147 to 388 with wlative GMT of 256. Correlation between concentration of IgY and AIB antibody titres was puted to be 0.88.

DOI

10.21608/vmjg.1997.378349

Authors

First Name

K

Last Name

SHAFIOQ

MiddleName

-

Affiliation

Department of Vet. Microbiology, University of Agriculture, Faisalabad-38040, Pakistan.

Email

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City

-

Orcid

-

First Name

H

Last Name

AFZAL

MiddleName

-

Affiliation

Department of Vet. Microbiology, University of Agriculture, Faisalabad-38040, Pakistan.

Email

-

City

-

Orcid

-

First Name

N

Last Name

KHANUM

MiddleName

-

Affiliation

Department of Vet. Microbiology, University of Agriculture, Faisalabad-38040, Pakistan.

Email

-

City

-

Orcid

-

First Name

M

Last Name

ARSHAD

MiddleName

-

Affiliation

Department of Vet. Microbiology, University of Agriculture, Faisalabad-38040, Pakistan.

Email

-

City

-

Orcid

-

Volume

45

Article Issue

3

Related Issue

50220

Issue Date

1997-07-01

Receive Date

2024-09-06

Publish Date

1997-07-01

Page Start

273

Page End

278

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_378349.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=378349

Order

378,349

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

EVALUOLATION OF EGG YOLK IMMUNOGLOBULINS (IgY) BY CHLOROFORM POLYETHYLENE GLYCOL TECHNIQUE AND ASSAYING OF ANTIBODIES AGAINST AVIAN INFECTIOUS BRONCHITIS

Details

Type

Article

Created At

23 Dec 2024