Two hundred samples, 100 each of raw and cooked beef were collected from different  restaurants in Benha. Samples were examined for rapid screening of E.coli by a fluorogenic assay.  E. coli was detected in 7% of fluorocult lauryl  sulphate enrichment broth which emits a blue fluorescence under UV light.The incidences of E. coli were 9% and 5% in examined raw and cooked beef respectively. O26: K60 (B6) and O111 : K58 (B4) were only the enteropathogenic serotypes isolated from the examined samples. The public health significance of isolated pathogens was discussed. Moreover, Suggestive measures for the improvement of the  microbial quality of meat were mentioned.