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377372

THE SWIFT DETECTION OF MYCOPLASMA CONTAMINATION IN CELL CULTURES BY USING POLYMYMERASE CHAIN REACTION

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Immunology / vaccine / diagnosis
Virology and viral diseases

Abstract

A total of 20 Samples (9 cultures, 7 sera and 4 medium samples) were used in this study. The stained culture samples showed pathological profile resembled that produced by Mycoplasmas. The cell aberrations, clumpings and the increased granularity came out as a result of the Myoplasma impairment influence on the amino acids, RNA and DNA synthesis of the cells which in turn reflected on their metabolic activities. Using the ordinary culturing technique, only 3 cultures and one serum sample gave the characteristic Mycoplasma colonies 4-6 weeks post culturing. The same samples with two cultures and 2 serum samples more were positive with Mycoplasma Polymerase Chain Reaction (PCR), The test was performed and the results were obtained in the same day. The positive bands on Agarose Gel Electrophoresis were of three sizes. A 600bp band seen in rabbit kidney culture samples which might be contaminated with M. orale or M. arginini. The second band (620bp) was observed in VERO, CEF and MDBK cultures that most likely infected with M.hyorhinis or M. fermentans. The third band was 100 bp noticed in the positve serum samples that most probably contaminated with Acholeplasma laidlauii. It could be concluded that PCR technique proved to be accurate, reliable and saving time & can be relied on to detect mycoplasma contamination of cell cultures. Further investigations should be carried out in order to make use of the set in screening of either conventionally produced or imported TC and egg adapted vaccines.

DOI

10.21608/vmjg.1998.377372

Authors

First Name

A.H.M.

Last Name

HUSSKIN

MiddleName

-

Affiliation

Vet Serum Vaccine Research Institute, Agriculture Research Center, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

SEHAM, A. S.

Last Name

EL-ZEEDY

MiddleName

-

Affiliation

Vet Serum Vaccine Research Institute, Agriculture Research Center, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

FADIA

Last Name

S.H. FANMEY

MiddleName

-

Affiliation

Vet Serum Vaccine Research Institute, Agriculture Research Center, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

A. M.

Last Name

DAOUD

MiddleName

-

Affiliation

Vet Serum Vaccine Research Institute, Agriculture Research Center, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

46

Article Issue

4

Related Issue

49902

Issue Date

1998-10-01

Receive Date

2024-08-31

Publish Date

1998-10-01

Page Start

773

Page End

783

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 46_Issue 4_Pages 773-783.pdf

PDF . 3.0MB

Link

https://vmjg.journals.ekb.eg/article_377372.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=377372

Order

377,372

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

THE SWIFT DETECTION OF MYCOPLASMA CONTAMINATION IN CELL CULTURES BY USING POLYMYMERASE CHAIN REACTION

Details

Type

Article

Created At

23 Dec 2024