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377364

ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) FOR DETECTION OF BVD VIRUS ANTIGEN IN BUFFY COATS OF MUCOSAL DISEASE AFFECTED CATTLE

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Veterinary medicine / clinical pathology

Abstract

Enzyme linked immunosorbent assay (ELISA) in comparison with tissue culture method was used for detection of bovine viral diarrhea virus (BVDH) antigen in 70 out 152 buffy coats from Mucosal disease affected cattle which were submitted to cattle clinic in Justus Liebig University, Giessen, Germany from 1994 to 1996. ELSA and tissue culture methods were performed in the Institute of Animal Hygiene and infectious Diseases, Justus Liebig University, Giessen, Germany. The results showed that, 58 (82.9%) and 61 (87.1) of buffy coats were positive in cell culture and direct ELISA respectively. All samples which were positive in cell culture were also positive in ELISA. Three cattle with chronic mucosal disease were positive with direct ELISA, but negative with cell culture method. In conclusion, diagnosis of mucosal disease by isolation and detection of BVD virus using tissue culture method is a time consuming technique. Direct ELISA is a sensitive, rapid, but expensive method for the detection of BVD virus antigen in comparison to the cell technique.

DOI

10.21608/vmjg.1998.377364

Authors

First Name

I.H.A.

Last Name

ABD EL-RAHIM

MiddleName

-

Affiliation

Members of Animal Medicine Department, Assiut University, Assiut, Egypt.

Email

-

City

-

Orcid

-

First Name

T. A. A.

Last Name

EL-ALLAWY

MiddleName

-

Affiliation

Professor of Internal Medicine & Director Ruminants Clinic, Justus- Liebig University, Giessen ,Germany

Email

-

City

-

Orcid

-

First Name

LAILA S.

Last Name

AHMED

MiddleName

-

Affiliation

Members of Animal Medicine Department, Assiut University, Assiut, Egypt.

Email

-

City

-

Orcid

-

First Name

H.D

Last Name

GRINDER

MiddleName

-

Affiliation

Professor of Internal Medicine & Director Ruminants Clinic, Justus- Liebig University, Giessen ,Germany

Email

-

City

-

Orcid

-

Volume

46

Article Issue

4

Related Issue

49902

Issue Date

1998-10-01

Receive Date

2024-08-31

Publish Date

1998-10-01

Page Start

701

Page End

708

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 46_Issue 4_Pages 701-708.pdf

PDF . 3.3MB

Link

https://vmjg.journals.ekb.eg/article_377364.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=377364

Order

377,364

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) FOR DETECTION OF BVD VIRUS ANTIGEN IN BUFFY COATS OF MUCOSAL DISEASE AFFECTED CATTLE

Details

Type

Article

Created At

23 Dec 2024