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377331

POLYMERASE CHAIN REACTION FOR CHARACTERIZATION OF VP4 GENE TYPES OF GROUP A BOVINE ROTAVIRUS DIRECTLY IN THE FECES OF NEONATAL DAIRY CALVES

Article

Last updated: 03 Jan 2025

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Tags

Virology and viral diseases

Abstract

A PCR (polymerase chain reaction) genotyping assay was applied to identify bovine rotavirus (BRV) gene 4 types (P genotypes, NCDV (PI), UK (P5), and B223 (P11) in the field samples collected from calves with and without diarrhoea. Rotavirus double Stranded RNAs (dsRNAs) extracted from the fecal samples were reverse transcribed and amplitied by PCR using two nucleotide primers that correspond highly conserved regions of the VP4+ gene among rotaviruses. The amplified products (that included the VP8 portion and the connecting peptide of the VP4 gene) were then amplified by PCR int he Presence of coctail of specific primers for three reference BRV. The PCR-typing assay was applied to identify BRV P genotypes (PI, P5 and P11) in 73 field samples. These samples were reacted positive for BRV by polyacrylamide gel electrophoresis and monoclonal antibody - based ELISA, The PCR assay was able to identify BRV Different P genotypes in 79.4% of the samples. Mix of two P genotypes was efficiently detected in The same samples by applied method .in order to detect all G/P possible combination types. among BRV field isolates, the P typing results were compared with the previously reported G-typing assay applied in this study provided a very sensitive method for genotyping of Brv field strains.

DOI

10.21608/vmjg.1998.377331

Authors

First Name

HUSSEIN A.

Last Name

HUSSEIN

MiddleName

-

Affiliation

Department of Virology, faculty of veterinary medicine, Cairo university, Giza 1221 , Egypt

Email

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City

-

Orcid

-

First Name

A.

Last Name

SILIM

MiddleName

-

Affiliation

Department of Virology, faculty of veterinary medicine, university of montreal, st-Hyacinthe ,Quebec J25 7C6, Canada

Email

-

City

-

Orcid

-

First Name

M.A

Last Name

SHALABY

MiddleName

-

Affiliation

Department of Virology, faculty of veterinary medicine, Cairo university, Giza 1221 , Egypt

Email

-

City

-

Orcid

-

First Name

Y.

Last Name

EL-AZHARY

MiddleName

-

Affiliation

Department of Virology, faculty of veterinary medicine, university of montreal, st-Hyacinthe ,Quebec J25 7C6, Canada

Email

-

City

-

Orcid

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Volume

46

Article Issue

4

Related Issue

49902

Issue Date

1998-10-01

Receive Date

2024-08-30

Publish Date

1998-10-01

Page Start

471

Page End

478

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 46_Issue 4_Pages 471-478.pdf

PDF . 2.7MB

Link

https://vmjg.journals.ekb.eg/article_377331.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=377331

Order

377,331

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

POLYMERASE CHAIN REACTION FOR CHARACTERIZATION OF VP4 GENE TYPES OF GROUP A BOVINE ROTAVIRUS DIRECTLY IN THE FECES OF NEONATAL DAIRY CALVES

Details

Type

Article

Created At

23 Dec 2024