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377330

DETECTION OF BOVINE HERPES NIRUS TYPE 1 IN SEMEN USING A RAPID AND SENSITIVE POLYMERASE CHAIN REAACTION ASSAY

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Theriogenology / reproductive technology

Abstract

polymerase Chain Reaction (PCR) for the detection of bovine herpesvirus type1 | (BHV-1) was evaluated. Since bovine semen contains components that inhibit PCR amplification, a protocol was used to purify BHV-1 DNA from semen. The study to compare the PCR assay with the routinely used virus isolation method showed that the PCR assay is 100-fold more sensitive. In addition, the results of the PCR assay are available in 1 day, whereas the virus isolation method takes 10-17 days. It is concluded that the PCR assay is the most sensitive technique for laboratory detection of BHV-I.

DOI

10.21608/vmjg.1998.377330

Authors

First Name

AMIN

Last Name

A.S.

MiddleName

-

Affiliation

Animal Reproduction Research Institute, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

IBRAHIM

Last Name

A.K.

MiddleName

-

Affiliation

Fac. Vet. Med.,Cairo Univ.

Email

-

City

-

Orcid

-

Volume

46

Article Issue

4

Related Issue

49902

Issue Date

1998-10-01

Receive Date

2024-08-30

Publish Date

1998-10-01

Page Start

463

Page End

469

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 46_Issue 4_Pages 463-469.pdf

PDF . 2.4MB

Link

https://vmjg.journals.ekb.eg/article_377330.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=377330

Order

377,330

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

DETECTION OF BOVINE HERPES NIRUS TYPE 1 IN SEMEN USING A RAPID AND SENSITIVE POLYMERASE CHAIN REAACTION ASSAY

Details

Type

Article

Created At

23 Dec 2024