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377324

DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA BY THE POLYMERASE CHAIN REACTION (PCR) IN FIFLD SAMPLES

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Theriogenology / reproductive technology

Abstract

Pasteurella multocida is an important cause of pneumonia in newly born animals, usually as a secondary pathogen invading lungs injured by other bacteria OF viruses. Toxigenic and notoxigenic Pasteurella multocida isolates can not be differentiated by morphology oF standard biochemical reaction. A more rapid and accurate method to detect toxigenic Pasteurella multocida is needed for improving clinical diagnosis, prevention , treatment and epidemiological studies. the feasibility of using polymerase chain reaction (PCR) amplification assay for accurate and rapid detection of toxigenic Pasteurella multocida in field samples was studied. The results showed that PCR assay could detect toxigenic Pasteurella Multocida antigen in field samples. The result Indicate that there was complete agreement of the Toxigenic status of the Pasteurella multocida Based on mouse lethality and PCR assay.the Concordance of PCR results with the defined toxigenic status indicates 100% specificity and sensitivity.The PCR protocol could be completed within one working day which is faster and more sensitive than the time consuming conventional diagnostic techniques currently used in clinical laboratories.

DOI

10.21608/vmjg.1998.377324

Authors

First Name

AMIN

Last Name

A.S

MiddleName

-

Affiliation

Animal Reproduction Research Institute, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

EL-BATRAWY

Last Name

N.E.

MiddleName

-

Affiliation

Animal Reproduction Research Institute, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

TANIOS

Last Name

M A.I.

MiddleName

-

Affiliation

Animal Health Research Institute, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

ATTIA

Last Name

E.R.J.

MiddleName

-

Affiliation

Animal Reproduction Research Institute, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

SHAABAN

Last Name

A.I.

MiddleName

-

Affiliation

Animal Health Research Institute, Giza, Egypt.

Email

-

City

-

Orcid

-

Volume

46

Article Issue

4

Related Issue

49902

Issue Date

1998-10-01

Receive Date

2024-08-30

Publish Date

1998-10-01

Page Start

443

Page End

449

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 46_Issue 4_Pages 443-449.pdf

PDF . 2.2MB

Link

https://vmjg.journals.ekb.eg/article_377324.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=377324

Order

377,324

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA BY THE POLYMERASE CHAIN REACTION (PCR) IN FIFLD SAMPLES

Details

Type

Article

Created At

23 Dec 2024