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377318

CLINICAL , LABORATORY AND MOLECULAR EPIDEMOLOGICAL STUDIES ON A LIMITED OUTBREAK OF ENZOOTIC BOVINE LEUCOSIS IN UPPER EGYPT

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Pathology / oncology
Veterinary medicine / clinical pathology

Abstract

In 1989,220 Holstein Friesian cattle (212 heifers and 8 bulls) were imported from Minnesota, USA to form a closed dairy herd in Arab El-Aoumar, Assiut, Upper Egypt. In November 1996 the population had built up to 440 animals. At that time some abnormal signs such as loss of weight, decreased milk yield, external lymphadenopathy and decreased appetite were observed in this farm. Serological screening by Enzyme linked immunosorbent assey (ELISA) revealed a seroprevalence of antibodies directed against Bovine Leucosis Virus (BLV) of 37.7% in cattle under 2 years old "of 72.8% j in animals more than two years old. Diagnosis was confirmed by the detection of BLV proviral DNA by polymerase chain reaction (PCR) using primers, amplifying a fragment of the env gene. Out of 22 tested leucocyte fraction from individual animals, 15 were positive showing a BLV-specific amplicon of 440 bp. The analysis of the amplicon for restriction fragment length polymorphisms and DNA sequencing results allowed to type the isolates. Since this is the first recorded case of enzootic bovie leucosis in upper Egypt , strict quaratine measures were adopted and all serologic positive animals of the herd culled.

DOI

10.21608/vmjg.1998.377318

Authors

First Name

A.

Last Name

ZAGHAWA

MiddleName

-

Affiliation

Department of Animal and Forensic Medicine, Faculty of Veterinary Medicine Alexandria University, Egypt

Email

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City

-

Orcid

-

First Name

I.H.A

Last Name

ABD EL-RAHIM

MiddleName

-

Affiliation

Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Egypt

Email

-

City

-

Orcid

-

First Name

S.

Last Name

EL -BALLAL

MiddleName

-

Affiliation

Department of Pathology, Faculty of Veterinary Medicine, Assiut University, Egypt

Email

-

City

-

Orcid

-

First Name

F.J

Last Name

CONTRATHS

MiddleName

-

Affiliation

Federal Research Center for Virus Diseases of Animals, Institute for Epidemiological Diagnstic , Wusterhausen, Germany

Email

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City

-

Orcid

-

First Name

O.

Last Name

MARQUART

MiddleName

-

Affiliation

Federal Research Center for Virus Diseases of Animals, Institute for Microbiology, Ubingen, Germany.

Email

-

City

-

Orcid

-

First Name

D.

Last Name

BEIER

MiddleName

-

Affiliation

Federal Research Center for Virus Diseases of Animals, Institute for Epidemiological Diagnstic , Wusterhausen, Germany

Email

-

City

-

Orcid

-

Volume

46

Article Issue

4

Related Issue

49902

Issue Date

1998-10-01

Receive Date

2024-08-30

Publish Date

1998-10-01

Page Start

393

Page End

405

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 46_Issue 4_Pages 393-405.pdf

PDF . 4.1MB

Link

https://vmjg.journals.ekb.eg/article_377318.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=377318

Order

377,318

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

CLINICAL , LABORATORY AND MOLECULAR EPIDEMOLOGICAL STUDIES ON A LIMITED OUTBREAK OF ENZOOTIC BOVINE LEUCOSIS IN UPPER EGYPT

Details

Type

Article

Created At

23 Dec 2024