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377285

ELECTRORETIC DIFFERENTIATION OF SOLUBLE ANTIGENS FROM ECHINOCOCCUS GRANULOSUS ISOLATES USING SDS-PAGE TECHNIQUE

Article

Last updated: 23 Dec 2024

Subjects

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Tags

Parasitology and parasitic diseases

Abstract

a comparison Was made by sodium dodecyle polyacrylamide gel electrophoresis SDS-PAGE between somatic hydatid fluid, Protoscoleces and Echinococcus granulous adults antigens recovered from camel and equine (donkey) origins. Comparing hydatid fluids indicated that differences were found in the arrangement and molecular weights of developed protein bands. Donkey hydatid fluid showed 3 protein bands having a molecular weight ranging between 44.6-143 K Da. while that of camel origin revealed 7 bands. These bands had variable molecular weights either low (28.8 - 46.7 K Da.) or high (72.4 - 100.0 K Da.). The protein patterns of protoscoleces were most complex in both origins, Protein experession in camel Protoscoleces had 12 bands. These bands had Variable molecular weights, 8 bands having low molecular weights (19.9 - 64.6 K Da.), the other 4 bands had higher molecular weights (89.1 - 136.3 K Da.). The identified bands among donkey protoscoleces were 8 bands. Only one band had a low molecular weight (53.7 K Da.). while the others were of high molecular weights ‘67.6 - 157.9 K Da.). Protein profile of E. granulosus somatic antigens revealed that both isolates had equal number of protein bands. All the stained protein bands were relatively similar except two bands having low molecular weight 10.5 & -6.3 K Da. However, one band characterized camel sample had a high molecular weight (105.3 K Da.).  

DOI

10.21608/vmjg.1998.377285

Authors

First Name

A.A.

Last Name

DERBALA

MiddleName

-

Affiliation

Parasitology and Animal Diseases Dept., National Research Centre, Dokki. Giza, Egypt.

Email

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City

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Orcid

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Volume

46

Article Issue

3

Related Issue

49901

Issue Date

1998-07-01

Receive Date

2024-08-30

Publish Date

1998-07-01

Page Start

285

Page End

292

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_377285.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=377285

Order

377,285

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

ELECTRORETIC DIFFERENTIATION OF SOLUBLE ANTIGENS FROM ECHINOCOCCUS GRANULOSUS ISOLATES USING SDS-PAGE TECHNIQUE

Details

Type

Article

Created At

23 Dec 2024