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372191

MYCOPLASMA AS CONTAMINANT OF CELL QULTURE WITH SPECIAL REFERENCE TO SOURCE OF INFECTION

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Bacteriology and bacterial diseases

Abstract

Nine tissue cultures, 7 media for virus propagation and 5 lots of bovine sera as enrichment were brought to the Mycoplasma research department and routinely examined for contamination with Mycoplasma Four M. hominis were isolated from cell cultures, 3 M. salivarium were isolated from culture media while bovine serum samples revealed 3 bovine mycoplasmas isolates, 2 of them were M. arginini and one as A. Laidlawii. Antibiotic sensitivity test for Mycoplasma isolates was applied against 8 antimicrobial agents using metabolic inhibition test (MIT) and growth inhibition test (GIT) and spectinomycin proved to be the most effective and was recommended for treatment of contaminated tissue cultures. A trial was made to investigate the source of contamination. Out of 10 laboratory personnel 7 (.70%) Mycoplasma salivanum were isolated from throat samples while 6 (60%) were isolated when they were allowed to talk in front of plates held 2 inches from the mouth.

DOI

10.21608/vmjg.1992.372191

Authors

First Name

LAILA

Last Name

EL-SHABINY

MiddleName

-

Affiliation

Animal Health Research Inst. Doki Giza.

Email

-

City

-

Orcid

-

First Name

IKRAM

Last Name

KARIM

MiddleName

-

Affiliation

Animal Health Research Inst. Doki Giza.

Email

-

City

-

Orcid

-

First Name

EL-NAKASHL

Last Name

S

MiddleName

-

Affiliation

Animal Health Research Inst. Doki Giza.

Email

-

City

-

Orcid

-

Volume

40

Article Issue

2

Related Issue

49631

Issue Date

1992-04-01

Receive Date

2024-08-06

Publish Date

1992-04-01

Page Start

43

Page End

47

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 40_Issue 2_Pages 43-47.pdf

PDF . 1.3MB

Link

https://vmjg.journals.ekb.eg/article_372191.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=372191

Order

372,191

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

MYCOPLASMA AS CONTAMINANT OF CELL QULTURE WITH SPECIAL REFERENCE TO SOURCE OF INFECTION

Details

Type

Article

Created At

23 Dec 2024