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369387

Isolation and identification of Egypt/Beni-Seuf/01: a novel genotype of infectious bronchitis virus

Article

Last updated: 03 Jan 2025

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Tags

Virology and viral diseases

Abstract

  Infectious bronchitis virus (IBV) strain Egypt/Beni-Seuf/01 was isolated from 38-day-old broiler chickens suffering from respiratory and renal disease in the Beni-Seuf governorate of upper Egypt in 1998. The strain was identified using electron microscopy (EM), agar gel precipitation test (AGPT), and reverse transcriptase polymerase chain reaction (RT-PCR). SI gene cycle sequencing of the RT-PCR product was used to determine the genotype of the strain. Three weeks old commercial ‘chickens were vaccinated with the IBV vaccine (H 120) and the protection level against challenge Egypt/Beni-Seuf/01 was evaluated via viral isolation attempts from tracheal scraping suspensions as well as tracheal histopathology at 4 days post-challenge. Kidney histopathology at 4 days post-challenge was examined. AGPT on the chorioallantoic membrane(CAM) homogenate of each passage using rabbit anti-IBV polyclonal serum showed positive results with all egg passages (Ist to 7th ). E/M examination revealed pleomorphic or round virions with widely spaced surface projections and 160nm average diameter. Alignment of the S1 sequence of Egypt\Beni-Seuf/01 with 22 published IBV strains demonstrated that the newly deposited sequence of Israel/720/99 is closely related to the Egyptian isolate. Vaccination of broiler-type chickens with the live H120 strain offered only 20% protection against challenges with the Egyptian strain. Kidney histo-pathological lesions indicated that Egypt\Beni-Seuf/Ol is a nephrogenic IBV. Results of SI sequencing and the vaccination trial indicate that the Egypt\Beni-Seuf/01 strain is a novel genotype for which H120 vaccination does not provide pro-infection.

DOI

10.21608/vmjg.2002.369387

Authors

First Name

S

Last Name

Abdelmoneim

MiddleName

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Affiliation

Department of virology, Faculty of Veterinary Medicine, Cairo University Beni-Seuf branch

Email

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City

-

Orcid

-

First Name

H

Last Name

Madbouly

MiddleName

-

Affiliation

Department of virology, Faculty of Veterinary Medicine, Cairo University Beni-Seuf branch

Email

-

City

-

Orcid

-

First Name

J

Last Name

Gelb

MiddleName

-

Affiliation

Department of Animal and Food Science, University of Delaware

Email

-

City

-

Orcid

-

First Name

B

Last Name

Ladman

MiddleName

-

Affiliation

Department of Animal and Food Science, University of Delaware

Email

-

City

-

Orcid

-

Volume

50

Article Issue

4

Related Issue

49349

Issue Date

2002-10-01

Receive Date

2024-07-26

Publish Date

2002-10-01

Page Start

1,056

Page End

1,078

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 50_Issue 4_Pages 1056-1078.pdf

PDF . 3.9MB

Link

https://vmjg.journals.ekb.eg/article_369387.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=369387

Order

369,387

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Isolation and identification of Egypt/Beni-Seuf/01: a novel genotype of infectious bronchitis virus

Details

Type

Article

Created At

23 Dec 2024