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368822

Study on fungal contamination of some chicken meat products with special reference to the use of PCR for its identification

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

Food safety / meat quality

Abstract

This work was carried out to evaluate the fungal contamination of chicken meat products sold in local markets as well as identification of some isolated moulds using PCR technique. For identification of the isolated moulds, samples were subjected to mycological examination using the morphological (macroscopic and microscopic) characterization. Molecular identification using (ITS) was carried out for isolated Aspergillus and Penicillium species. The average total mould counts in the examined samples of chicken luncheon, chicken pane and chicken minced meat were 3.1 x 102±0.82 x 102, 7.4x 102± 5.4 x 102and 1.7 x 102 ± 0.16 x 102cfu/gm, respectively. In the examined samples, 9 mould genera were identified. The identified mould genera were Aspergillus, Eurotium, Penicillium, Geotrichum, Fusarium, Cladosporium, Mucor, Eupencillium and Acremonium species. The isolated species of Aspergillus parasiticus and Penicillium purpurogenum were subjected to PCR identification, and were sequenced in both directions. Sequences were analysed and aligned by Clustal method using the program of DNAstar (Laser-gene, Wisconsin, USA).

DOI

10.21608/vmjg.2014.368822

Keywords

A. parasiticus, Chicken meat, PCR, sequences, P. purpurogenum

Authors

First Name

Shaltout

Last Name

Aziz

MiddleName

-

Affiliation

Department of Food Hygiene, Faculty of Vet. Med. Moshtohor Benha University

Email

-

City

-

Orcid

-

First Name

El-diasty

Last Name

Mahmoud

MiddleName

-

Affiliation

Department of Mycology, Animal Health Research Institute Dokki, Giza

Email

-

City

-

Orcid

-

First Name

Manal

Last Name

El-mesalamy

MiddleName

-

Affiliation

Zagazig Provincial Lab, Department of Mycology, Animal Health Research Institute

Email

-

City

-

Orcid

-

First Name

Manal

Last Name

El-shaer

MiddleName

-

Affiliation

Zagazig Provincial Lab, Department of Food Hygiene, Animal Health Research Institute

Email

-

City

-

Orcid

-

Volume

60

Article Issue

2

Related Issue

49339

Issue Date

2014-04-01

Receive Date

2024-07-22

Publish Date

2014-04-01

Page Start

1

Page End

22

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_368822.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368822

Order

368,822

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Study on fungal contamination of some chicken meat products with special reference to the use of PCR for its identification

Details

Type

Article

Created At

23 Dec 2024