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368354

Optimization of polymerase chain reaction for direct detection of colibacillosis in infected chicken

Article

Last updated: 03 Jan 2025

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-

Tags

Bacteriology and bacterial diseases

Abstract

  A total of 33 local E. coli isolates were in this study. These isolates were biochemically and serologically identified as O1, O2, O6, O78 and O126. Four sets of oligonucleotide primer sequences were designed specifically for 16S-RNA, STX, Sth and eaeA genes. DNA and Plasmids were extracted and polymerase chain reaction was optimized for each. 16SrRNA gene primer successfully amplified with all serotypes giving rise a product mass of 204 bp, while STX gene primer was amplified with O1, O2 and O78 types in a specific band at 323 bp. At the same time the specific primers of Sth gene get a 171 bp molecular weight product only with O6 type meanwhile the eaeA gene primers successfully amplified only with O126 serotype giving rise a molecular weight band at 200 bp. In conclusion, PCR assay was able to differentiate between the different serotypes of E. coli in the suspected samples saving time, money and effort.

DOI

10.21608/vmjg.2011.368354

Authors

First Name

El-Seedy

Last Name

R

MiddleName

-

Affiliation

Dept.of Bacteriology, Mycology and Immunology, Faculty of Vet. Med, Beni-Suef University

Email

-

City

-

Orcid

-

First Name

Hassan

Last Name

H

MiddleName

-

Affiliation

Dept.of Bacteriology, Mycology and Immunology, Faculty of Vet. Med, Beni-Suef University

Email

-

City

-

Orcid

-

First Name

Salama

Last Name

S

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics

Email

-

City

-

Orcid

-

First Name

Fatma El-Zahraa

Last Name

Gamal

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics

Email

-

City

-

Orcid

-

Volume

59

Article Issue

4

Related Issue

49194

Issue Date

2011-10-01

Receive Date

2024-07-21

Publish Date

2011-10-01

Page Start

307

Page End

318

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 59_Issue 4_Pages 307-318.pdf

PDF . 1.9MB

Link

https://vmjg.journals.ekb.eg/article_368354.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368354

Order

368,354

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Optimization of polymerase chain reaction for direct detection of colibacillosis in infected chicken

Details

Type

Article

Created At

23 Dec 2024