The main objectives of the present work are to study the effect of treated sesame seeds (Sesamum Indicium L) with roasting at 180°c for 15 min. and exposure to gamma irradiation at dose level of 2.5, 5 and 10 KGy on the antinutritional factors as well as evaluation of some biochemical analysis. In the present study groups of 10 rats were assigned to receive one of six experimental diets (i.e. raw roasted and irradiated sesame seeds up to 10 KGy diet, along side casein diet). The statistical analysis indicated that there were significant results (p<0.05) decreased in the trypsin inhibitors, tannins of roasting and irradiated sesame seeds. There were no Significant differences in the phenol compounds of sesame seeds processed compared with raw samples. The obtained data showed that the level of total serum protein and albumin were significantly lowered in rats fed raw sesame seeds (5.24 and 4.15 g dl^-1)   as compared with those fed casein diet (6.03 g dI ^-1). Moreover, the rises of total serum protein of irradiation processing of sesame seeds up to 10 KGy was successful due to reducing the antinutritional factors by increasing irradiation process, With respect to the total serum albumin in rats fed irradiated sesame seeds at 5 and 10 KGy was close to that rats fed casein diet, followed with significant (p<0.05) decrease in the group of rats received in their diets irradiated sesame seeds at 2.5 KGy: roasting and raw samples when compared with those fed casein diet. The statistical analysis showed that there were no significant variation in the liver enzymes (ALT and AST) between rats fed dry heated sesame seeds and those fed raw samples. But the level of liver enzymes (ALT and AST) decreased when rats fed sesame seeds irradiated up to 10 KGy as compared with rats fed raw seeds. Results showed significant decrease in total cholesterol, triacylglycerol and low. density lipoprotein cholesterol (LDL-C) and the results reflected increase in the high density lipoprotein (HDL.-C) for rats received dry heated and irradiated (up to 10 KGy) sesame seeds when compared with those fed raw samples.