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354952

Isolation and Characterization of Avian Reovirus (Egypt/ARV/Giza 2011) Associated with Arthritis in Broiler Breeder Flocks

Article

Last updated: 23 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Avian diseases / production
Virology and viral diseases

Abstract

Samples of tibiotarsal tendons, tendon sheaths and fluids around ruptured tendons were collected from broiler breeder flock of 28 weeks old in Giza governorate, showed symptoms of arthritis, lameness and swelling in the hock joint. Trial for isolation of avian reovirus (ARV) was done by inoculation in embryonated chicken egg (ECE). After propagation and titration of the isolated virus, it had beed antigenically characterized by agar gel precipitation test (AGPT) and fluorescent antibody technique (FAT). Pock lesions on the chorioallantoic membrane (CAM) was negatively stained and examined by electron microscope, also histopathological examination was carried out. Molecular characterization was performed by reverse transcriptase polymerase chain reaction (RT.PCR). The results confirm the isolation of avian reovirus from the suspected flocks

DOI

10.21608/vmjg.2024.354952

Keywords

avian, Reovirus, Arthritis, CAM and pock

Authors

First Name

Latifa

Last Name

Bekheet

MiddleName

-

Affiliation

Specific Pathogen Free Eggs Project, Koum Oshiem, El-Fayoum Governorate

Email

-

City

-

Orcid

-

First Name

Hussein

Last Name

Hussein

MiddleName

A

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Cairo University

Email

husvirol@cu.edu.eg

City

-

Orcid

-

First Name

M.M

Last Name

Elsafty

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, VSVRI, Abbasia, Cairo

Email

-

City

-

Orcid

-

First Name

L.A.

Last Name

Tantawy

MiddleName

-

Affiliation

Animal Health Researches Institute, AHRI, Dokki, Giza

Email

-

City

-

Orcid

-

First Name

A.M.

Last Name

Helal

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, VSVRI, Abbasia, Cairo

Email

-

City

-

Orcid

-

Volume

63

Article Issue

1

Related Issue

47592

Issue Date

2017-01-01

Receive Date

2024-05-17

Publish Date

2024-05-17

Page Start

75

Page End

81

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 63_Issue 1_Pages 75-81.pdf

PDF . 4.3MB

Link

https://vmjg.journals.ekb.eg/article_354952.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=354952

Order

354,952

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Isolation and Characterization of Avian Reovirus (Egypt/ARV/Giza 2011) Associated with Arthritis in Broiler Breeder Flocks

Details

Type

Article

Created At

23 Dec 2024