Bilosomal are considered as nano structures with bile salts incorporated into the lipid bilayer of the membrane are abundant. Drug-loaded bilosomes have received considerable attention as drug delivery systems for a variety of drugs. The properties of bile salts are crucial in drug delivery systems. One of the most fascinating research areas nowadays is the use of bilosomes to produce biotechnology products and innovative vaccines. thus,somes have thus been developed as a potential vesicular carrier system for oral vaccine delivery. Besides oral vaccination, oral drugs susceptible to lysis by natural bile salts can be incorporated into bilosomes. The possibility of using bilosomes for transdermal, ocular, and intranasal applications is a further benefit. The current article also discusses bilosome composition and formulation techniques. The impact of newly employed nonionic surfactants and the physicochemical properties of bile salts on the formulation of bilosomes as well as their benefits over traditional nanocarriers are also covered.