Keywords: Agarose- chitosan- Emdogain- biomimetic remineralization. Objectives: The purpose of the study was to compare the efficiency of different agarose-based hydrogels in the treatment of dentin erosion. Methodology: Eighteen human molars were collected. Radicular part of each molar was removed, while coronal part was sectioned mesiodistally. Two dentin slabs were obtained from each molar. Dentin specimens were immersed in Coca Cola beverage (Coca-Cola® Co., Egypt) for 25 hours. Specimens in group I were stored in artificial saliva (control), in group II they were treated with agarose hydrogel system, in group III chitosan was added, and in group IV agarose hydrogel system was loaded with Emdogain. Hydrogels were applied to specimens for 5 hours. This procedure was repeated daily for 15 days. Morphology of all specimens was investigated by Scanning Electron Microscope. Results: A smear layer occluding some dentinal tubules was visible in images of sound dentin, while after immersion in Coca Cola images showed exposure of tubules' orifices. In group I, images revealed partially obliterated and opened tubules, while in group II they revealed partial obliteration of all tubules. Additionally, images of group III exhibited mild sclerosed dentinal tubules with enamel prism-like grown crystals, and in group IV presented regenerated crystals assembled into parallel bundles. Conclusion: Dentin erosion cannot be treated with artificial saliva. Agarose hydrogel group resulted in formation of enamel prism-like structures, Chitosan group showed densely packed crystal layer covering dentin surface, and Emdogain group revealed the formation of regenerated crystals and parallel bundles masking dentinal tubules openings.