Beta
ManageSearchChatDiscover
381853

Green Chromatographic Method for The Determination of Synthetic Azo Dyes in Processed Beef and Chicken Meat Products

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Pharmacology & Toxicology

Abstract

Food colorants in meat are controlled by European and non-European statutes for food safety concerns. A sensitive and eco-friendly method using High-Performance Liquid Chromatography (HPLC) with a diode-array detector and ammonia-ethanol extraction has been developed to accurately measure seven commonly used sulfonate azo colorants (Amaranth, Erythrosine, Sunset Yellow, Azorubin, Ponceau 4R, Red 2G, and Allura red) in processed meat samples within 10 min. Separation was successful using an Agilent ZORBAX RRHD Eclipse Plus C-18, 2.1 × 100 mm, 1.8 μm column at ambient temperature with a mobile phase consisting of 2.5 mL of Triton X-100 up to 1 L with phosphate buffer solution (0.05 M) at pH 7. The greenness score was measured using the AGREE approach. This highly sensitive and specific method makes it suitable for the analysis of complex matrices. The method demonstrated good analytical performance in terms of sensitivity, selectivity, precision, and accuracy. Recoveries ranging from 92% to 102% were obtained, which met standard analytical requirements. The AGREE score of 0.82 indicates excellent greenness of the HPLC assay. These findings show that the method can effectively be used for conducting commercial meat product confirmation studies.

DOI

10.21608/ejvs.2024.293417.2132

Keywords

synthetic colorants, meat products, Chromatography, GAC

Authors

First Name

Maha,

Last Name

Abd-Elhafeez

MiddleName

S.

Affiliation

Chemistry, Toxicology and Feed Deficiency Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Giza 12619, Egypt.

Email

mahasabry86_doctor@hotmail.com

City

Giza

Orcid

0000-0002-5201-0214

First Name

Fatma

Last Name

Amro

MiddleName

H.

Affiliation

Reference laboratory of veterinary Quality Control on Poultry Production (RLQP), Animal Health Research Institute ( AHRI), Agriculture Research Center (ARC), Giza 12619, Egypt.

Email

fatmaamro_87@hotmail.com

City

Giza

Orcid

0000-0001-9193-8528

First Name

Mai

Last Name

Fadel

MiddleName

Aabd El-monem

Affiliation

Associate professor of pharmacology at the Pharmacology and Pyrogen Unit, Department of Chemistry, Toxicology and Feed Deficiency, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Giza, Egypt.

Email

dr.mai87@yahoo.com

City

cairo

Orcid

0000-0002-8440-439X

First Name

Mohamed

Last Name

Gaffer

MiddleName

H.

Affiliation

Food Hygiene Dep., Animal Health Research Institute, Agriculture Research Center of Egypt, Shebin El koom Branch, Egypt.

Email

mohamedhamdy_1980@yahoo.com

City

menofia

Orcid

0009-0003-5687-142X

First Name

Eman

Last Name

Elkhawaga

MiddleName

-

Affiliation

Food Hygiene Dept., Animal Health Research Institute, Mansoura, Agricultural Research Center, Egypt

Email

emanelkhawaga@yahoo.com

City

Egypt

Orcid

0009-0007-2818-498X

First Name

Eman

Last Name

Shukry

MiddleName

-

Affiliation

Animal health research institute

Email

emanshukry81@gmail.com

City

-

Orcid

0000-0002-0703-4516

First Name

Pierre

Last Name

Mehanny

MiddleName

E.

Affiliation

Biochemistry, Toxicology, Feed Deficiency Department, Animal Health Research Institute, Agricultural Research Center, Dokki, Giza 12618, Egypt

Email

pezzat7@gmail.com

City

Egypt

Orcid

0009-0000-3282-757X

First Name

Fady

Last Name

Youssef

MiddleName

Sayed

Affiliation

Department of Pharmacology, faculty of veterinary Medicine, Cairo university

Email

fadyalsalhany@cu.edu.eg

City

Giza

Orcid

0009-0008-2159-8837

Related Issue

-2

Receive Date

2024-05-29

Publish Date

2024-09-25

Page Start

1

Page End

11

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS3818531727211600.pdf

PDF . 887.1kB

Link

https://ejvs.journals.ekb.eg/article_381853.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=381853

Order

381,853

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Green Chromatographic Method for The Determination of Synthetic Azo Dyes in Processed Beef and Chicken Meat Products

Details

Type

Article

Created At

23 Dec 2024