Beta
ManageSearchChatDiscover
377659

In Vivo Investigation of the Anti-Staphylococcus aureus Impact of Balanites aegyptiaca and Curcuma longa Ethanol Extracts on Apoptotic Gene Expression and Their Implications on H

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Veterinary Parasitology & Microbiology

Abstract

We aim to determine effects of BAF-EE and CLP-EE ethanol extracts on apoptotic-gene expression , hemogram and serum protein profiles in S. aureus-infected mice. HPLC was utilized for qualitative estimation of phenolic compounds in both extracts. Sixty mice were divided into non-infected Group (n=15) and a group of 45 mice that were inoculated S/C with 0.1 ml of 2 ×107 CFU of a well-identified isolate of S. aureus. On 7th dpi, mice were subdivided into 3 equal groups and were orally dosed with BAF-EE, CLP-EE and Ciprofloxacin, at dosages of 200 mg, 100 mg and 20 mg/Kg BW/daily for 5 days, respectively. All mice were clinically monitored for 4 weeks for local inflammatory reactions and symptoms of sepsis. On days 3, 7, 14, 21, and 28, two blood samples were taken from three mice in each group before being sacrificed. Blood samples were used for hemogram and serum protein assays. The expression of apoptosis-genes in liver cells was investigated via semi-qRT-PCR. The data obtained show that four phenolic compounds were identified in each extract. Erytrogram was elevated after all treatments while, leucocyte counts except monocytes were elevated in mice treated with both extracts. Total protein and globulins increased in ciprofloxacin-treated mice. Treatment with both extracts reduced the expression of pro-apoptotic genes caspase-3, Bax and p53 while, increasing the anti-apoptotic gene Bcl-2, with CLP-EE outperforming BAF-EE. The study concludes that both CLP-EE and BAF-EE seem to have antibacterial capabilities, as evidenced by their protective impact on apoptosis-related genes and blood picture.

DOI

10.21608/ejvs.2024.302519.2240

Keywords

Staphylococcus aureus, Balanites aegyptiaca, Curcuma longa, apoptosis, Blood metabolic profile

Authors

First Name

Hala

Last Name

Abu zeina

MiddleName

A. A.

Affiliation

-

Email

hala_zeina60@yahoo.com

City

-

Orcid

0000-0001-7474-4596

First Name

Gamil

Last Name

Zeedan

MiddleName

SG

Affiliation

Department of Parasitology and Animal Diseases , National Research Centre, Giza, Egypt

Email

gamilzee@yahoo.com

City

Cairo

Orcid

-

First Name

Tamer

Last Name

Abd El-Aziz

MiddleName

H.

Affiliation

National Research centre,EGYPT

Email

tamer_ghname@yahoo.com

City

Giza

Orcid

-

First Name

Doaa

Last Name

Sedky

MiddleName

-

Affiliation

Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, 33 Bohouth Street, Dokki, P.O. Box 12622, Giza, Egypt.

Email

doaa_sedky@yahoo.com

City

Giza

Orcid

-

First Name

Amany

Last Name

Mohamed

MiddleName

-

Affiliation

Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, 33 Bohouth St., Dokki, 12622 Giza, Egypt.

Email

amanymmm2010@gmail.com

City

Cairo

Orcid

-

First Name

Abeer

Last Name

Abdalhamed

MiddleName

M.

Affiliation

Department of Parasitology and Animal Diseases, National Research Centre,Giza, Egypt

Email

abeerg2007@yahoo.com

City

Giza

Orcid

-

First Name

Sekena

Last Name

Abdel-Aziem

MiddleName

-

Affiliation

Cell Biology Department, National Research Centre,Giza, Egypt

Email

nadanahl@yahoo.com

City

Caro

Orcid

-

First Name

Manal

Last Name

Khafagi

MiddleName

-

Affiliation

Department of Parasitology and Animal Diseases, National Research Centre, Giza, Egypt

Email

manalhamdy41@yahoo.com

City

Caro

Orcid

-

First Name

Soad

Last Name

Nasr

MiddleName

M.

Affiliation

Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622, Giza, Egypt.

Email

soadnasr@yahoo.com

City

Giza, Egypt

Orcid

0000-0002-1538-8912

Related Issue

-2

Receive Date

2024-07-08

Publish Date

2024-09-01

Page Start

1

Page End

17

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS3776591725138000.pdf

PDF . 2.1MB

Link

https://ejvs.journals.ekb.eg/article_377659.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=377659

Order

377,659

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

In Vivo Investigation of the Anti-Staphylococcus aureus Impact of Balanites aegyptiaca and Curcuma longa Ethanol Extracts on Apoptotic Gene Expression and Their Implications on H

Details

Type

Article

Created At

23 Dec 2024