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355166

Detection of Escherichia coli and Associated Shiga Toxin Genes in Feces of Cattle and Sheep Reared in Egyptian Research Farm

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Veterinary Parasitology & Microbiology

Abstract

Ruminants are concerned as the prime reservoir for Shiga toxin-outputting Escherichia coli (STEC). Knowledge of STEC incidence in the live animal is substantial to constituting informed deductions about food safety. A total of 138 bacterial cultures (96 and 42 obtained from apparently healthy cattle and sheep feces respectively. A real time PCR was conducted using to detect E. coli species. On the other side, a conventional PCR was performed to determine amplification of Shiga toxin genes (stx1 and stx2). E. coli isolates were confirmed by real time PCR in all examined bacterial cultures (100%). Overall, (44.79%) of cattle isolates and (35.71%) of sheep ones were stx-positive. Thirty three (23.9%) of the isolates were stx1 positive only, 25 (18.12%) were stx2 only, while 13 (9.42%) carried both stx1 and stx2. Both apparently healthy cattle and sheep are asymptomatic reservoirs of STEC. Direct contact with these livestock or consumption of foodstuffs or water contaminated with their feces may constitute a potential source of infection for humans.

DOI

10.21608/ejvs.2024.273434.1881

Keywords

phoA, RT-PCR, ruminant, stx

Authors

First Name

Wahid

Last Name

El-Dabae

MiddleName

H.

Affiliation

Department of Microbiology and Immunology, Veterinary Research Institute, National Research Centre, Dokki, Giza, Egypt

Email

dr_wahidhussein@yahoo.com

City

CAIRO

Orcid

0000-0002-3506-2207

First Name

Sohier

Last Name

Syame

MiddleName

M.

Affiliation

Department of Microbiology and Immunology, Veterinary Research Institute, National Research Centre, Dokki, Giza, Egypt

Email

sohirsyam004@gmail.com

City

Cairo

Orcid

0000-0002-4335-7750

First Name

Elgabry

Last Name

Elgabry

MiddleName

Abdelalim

Affiliation

Department of Microbiology and Immunology, Veterinary Research Institute, National Research Centre, Dokki, Giza, EgyptNational Research Centre

Email

elgabry73@hotmail.com

City

Cairo

Orcid

0000-0002-0843-0035

First Name

Mona

Last Name

Soliman

MiddleName

M. H.

Affiliation

Department of Microbiology and Immunology, Veterinary Research Institute, National Research Centre, Dokki, Giza, Egypt

Email

mona_nrc.micro@yahoo.com

City

Cairo

Orcid

0000-0003-0871-0632

First Name

Engy

Last Name

Farahat

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Veterinary Research Institute, National Research Centre, Dokki, Giza, Egypt

Email

engyelsady@yahoo.com

City

Cairo

Orcid

0000-0001-8423-8056

Related Issue

-2

Receive Date

2024-02-28

Publish Date

2025-07-01

Page Start

1

Page End

6

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS3551661751317200.pdf

PDF . 1.2MB

Link

https://ejvs.journals.ekb.eg/article_355166.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=355166

Order

25

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Detection of Escherichia coli and Associated Shiga Toxin Genes in Feces of Cattle and Sheep Reared in Egyptian Research Farm

Details

Type

Article

Created At

23 Dec 2024