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Evaluation of The In vitro Inhibitory Effects of Etoposide On The Growth of Babesia and Theileria Parasites

Article

Last updated: 01 Jan 2025

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Tags

Veterinary Parasitology & Microbiology

Abstract

Etoposide exhibits antibacterial, antimalarial, and anticancer properties. Three Babesia parasites namely, B. bovis, B. bigemina, and B. caballi and Theileria equi were used to assess the remedial impacts of etoposide in vitro. Etoposide's impact on the transcription of the type II DNA topoisomerase, the DNA gyrase subunits A and B genes, has been studied using reverse-transcription PCR. Etoposide exhibited significant growth inhibition, having IC50 estimates of 3.5 for B. bovis, 4 for B. bigemina, 3.7 for B. caballi, and 4 for T. equi nM. It suppressed growth in B. bovis and T. equi, B. bigemina, and B. caballi at concentrations, including 25 µM and 10 µM, respectively. Throughout the viability assay, it inhibited the regrowth of the four piroplasms at 10 µM. RT-PCR revealed etoposide therapy repressed DNA gyrase transcription in B. bovis. Therefore, the DNA gyrase is the target of etoposide in B. bovis. Etoposide showed promise in treating babesiosis and theileriosis in vitro but requires further studies in vivo evaluation for mouse babesiosis.

DOI

10.21608/ejvs.2023.242050.1638

Keywords

Etoposide, in Vitro, RT-PCR, DNA Gyrase

Authors

First Name

Mahmoud

Last Name

AbouLaila

MiddleName

-

Affiliation

Department of Parasitology, Faculty of Veterinary Medicine, Damanhour University, Elgomhoria Street, Damanhour22511, Elbehera, Egypt

Email

mahmoud.aboulaila@vetmed.dmu.edu.eg

City

-

Orcid

0000-0003-1535-3696

First Name

Soad

Last Name

Menshawy

MiddleName

-

Affiliation

Department of Parasitology, Faculty of Veterinary Medicine, Damanhour University, Elgomhoria Street, Damanhour22511, Elbehera, Egypt

Email

soadelmenshawy@yahoo.com

City

Damanhour

Orcid

-

First Name

Besheer

Last Name

Elshafey

MiddleName

G.

Affiliation

Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, El-Behera, Egypt

Email

besheer_elshafey@vetmed.dmu.edu.eg

City

-

Orcid

-

First Name

Ahmed

Last Name

Osman

MiddleName

E.

Affiliation

Department of Biochemistry, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt

Email

ahmed-elsayd@vet.sohag.edu.eg

City

-

Orcid

-

First Name

Naoaki

Last Name

Yokoyama

MiddleName

-

Affiliation

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan

Email

yokayama@obihiro.ac.jp

City

-

Orcid

-

First Name

Ikuo

Last Name

Igarashi

MiddleName

-

Affiliation

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan

Email

igaracpmi@obihiro.ac.jp

City

-

Orcid

-

First Name

Amer

Last Name

Abdelaziz

MiddleName

R.

Affiliation

Department of Parasitology, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt

Email

amerragheb36@yahoo.com

City

-

Orcid

-

Volume

54

Article Issue

7

Related Issue

43917

Issue Date

2023-12-01

Receive Date

2023-10-11

Publish Date

2023-12-01

Page Start

211

Page End

219

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 54_Issue 7_Pages 211-219.pdf

PDF . 1.5MB

Link

https://ejvs.journals.ekb.eg/article_327429.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=327429

Order

327,429

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Evaluation of The In vitro Inhibitory Effects of Etoposide On The Growth of Babesia and Theileria Parasites

Details

Type

Article

Created At

23 Dec 2024