THIS study aimed to investigate the effect of in ovo injection of organic zinc and eggs subjected to high temperature (39.8^oC) during the late stage of incubation on post-hatch performance and immune response of local Egyptian strain (Bandarah) chicks. A total of 400 fertile eggs were weighed and randomly distributed to 8 groups with 5 replicates 10 eggs each. On the 14^th day of incubation, eggs were injected asT1: Eggs not injected (control), T2: eggs injected with 1ml saline solution, T3: eggs injected with 1ml saline solution containing 50µg Zn-Met, T4: eggs injected with a saline solution containing 100µg Zn-Met. Treatments from T1 to T4 incubated at normal temperature of 37.8^oc and 60%RH, treatments from T5 toT8 injected similar doses as described and subjected to high temperature of 39.8^oc when eggs are transferred from setter to hatcher machine for 3 hours at 18, 19, and 20 days of incubation. Results showed that in ovo injection with 50 and 100 µg Zn-Met/egg and subjected to high temperature during late incubation could improve hatching, chicks' weight, antioxidant activities, and increased WBC^,s and lymphocyte. Group injected with 100µg Zn-Met/egg was more effective in improving high-density lipoprotein, low-density lipoprotein, triglycerides, cholesterol, glucose, zinc, and T₃.