This work was aimed to investigate the epidemiology and methods of diagnosis and treatment of Salmonella paratyphoid infection in chickens during the period from March to December 2020. For this purpose, a total of 200 suspected diseased chicks 2-14 day old were collected from 10 chicken farms located in Kafrelsheikh Governorate, Egypt. These chicks were examined, and then samples were taken from lung, liver, intestine and gall bladder to investigate the current situation of the incidence of Salmonella paratyphoid. These samples were examined bacteriologically, biochemically and serologically and histopathological examination with treatment of experimentally infected chicks. The results revealed that 36 isolates with an incidence of (18%). The prevalence of Salmonella paratyphoid in the internal organs was highest in the intestine (55.6%), followed by liver (25%), gall bladder (13.9%), and lowest in lung (5.6%). Serotyping of Salmonella isolates revealed that two different serotypes (S. Kentucky and S. rechovot) with incidence of 28(77.8%) and 8(22.2%) respectively. The antibiogramme of isolated salmonella strains revealed sensitivity to thiamphenicol, amoxycillin and sulphatrimithoprine, respectively, but, resistant to flomoquine, neomycin and clindamycin.