In the past ten years, biomedical nanomaterials have attracted a lot of attention. Because of their extensive and significant biological features and biomedical uses, they have underlined several issues. There are many medical applications for metal oxide nanoparticles, including antibacterial, drug/gene delivery, anticancer, cell imaging, and biosensing. In recent years, a variety of industries, including the pharmaceutical, cosmetic, concrete, antibacterial, and textile industries, as well as the automotive industry, have used zinc oxide nanoparticles (ZnO NPs) as a major material. The potential of ZnO NPs to create reactionary oxygen species and initiate cell programmed (apoptosis) is associated with anticancer and antibacterial activities. So, in order to meet the increased demand for ZnO NPs, numerous synthetic techniques have been used to produce them. There is now a search for additional alternatives with environmental and financial advantages due to the economic and environmental costs associated with the majority of ZnO NP production methods. It's interesting that the biological technique of synthesis, which uses plants, plant extracts, or microbes as sources, has been proven suitable for the creation of ZnO NPs because of its multiple medical, health, environmental, and economic advantages. This review summarized the most current developments in the synthesis and biomedical uses of ZnO NPs made using green synthesis.