Cryptococcosis is infection with the fungi Cryptococcus neoformans, which may occur in the feces of many types of birds, and soil. The mean of study isolation of Cryptococcus neoformans from chicken and pet bird dropping sample in Mosul province. One hundred and twenty five samples were collected from dropping belong was of chicken (75) and pet bird (50). Cultured on Sabouraud Dextrose Agar (SDA) and Cryptococcus differential agar, isolates of Cryptococcus neoformans were diagnosed micromorphology by fluorescence microscope. The results revealed that the percentage of Cryptococcus neoformans isolation in chicken was 10/75 (13.3%), while in Pet bird was 15/50 (30%). All Cryptococcus neoformans strains grew on Cryptococcus differential agar at 37ᵒC for 2-5 days, cultural characteristic of positive isolates were observe as light blue. The Cryptococcus neoformans appear a positive acridine orange in the image its more sensitive to detection by being yellow against a dark background. The current study proves that the presence of C.neoformans in abundance as an environmental contaminant considered a potential source of infection for humans and animals in the Mosul province.