Beta
248606

Possible Synergistic Effect of Platelet Rich Plasma and Losartan on Experimentally Induced Skeletal Muscle Injury in Adult Male Albino Rats: A Histological and an Immunohistochem

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

-

Abstract

Introduction: Minimization of the disability after skeletal muscle injuries is still a challenge. Understanding of the contribution of growth factors has sparked a lot of interest in platelet-rich plasma therapy (PRP).Another line of treatment is the usage of anti-fibrotic agents as losartan.
Aim of the Work: Was to test the therapeutic effect of PRP, losartan and their combined treatment on skeletal muscle injury.
Materials and Methods: Forty two adult male albino rats were distributed into 2 groups: Group I (Control uninjured group) and Group ΙΙ (Treated injured rats' group). The latter was further subdivided into 4 equal subgroups and received: no treatment, single intramuscular injection of 100 μL PRP, single dose of 10 mg/kg/day losartan by oral gavage, and combined treatment of PRP and losartan respectively. The rats were sacrificed and light and electron microscopy were performed to examine the specimens of gastrocnemius muscle. In addition, immunohistochemical staining for CD 34 was performed.
Results: Subgroup IIA showed interrupted muscle fibers with focal areas of myofibrillar loss. Meanwhile, subgroups IIB and IIC revealed more regenerative changes. Evident hypercellularity with variable migrating cells were detected. In addition, neovascularization in some areas of subgroup IIC was noticed. Subgroup IID showed renewed muscle fibers in different stages with few structural changes. The results of the immuno-stained samples showed a positive reaction along the boundaries of some muscle fibers in subgroup IIA. Whereas the positive reaction in IIB, IIC and IID subgroups extended to the lateral borders and within some affected fibers and around the new pale nuclei, expressing advanced muscle regeneration.
Conclusions: After a muscle contusion, a combination of PRP and losartan treatment may enhance overall skeletal muscle recovery.

DOI

10.21608/ejh.2022.142908.1698

Keywords

PLATELET RICH PLASMA LOSARTAN SKELETAL MUSCLE

Authors

First Name

Amira

Last Name

Neanaa

MiddleName

Hashem

Affiliation

Assistant lecturer of Histology and Cell Biology, Faculty of Medicine, University of Alexandria.

Email

dr.mero_2014@hotmail.com

City

Alexandria

Orcid

0000-0001-9407-1157

First Name

Moushira

Last Name

El-Heneidy

MiddleName

-

Affiliation

Professor of Histology and Cell Biology Faculty of Medicine Alexandria University

Email

moushiraelhenneidy@hotmail.com

City

Alexandria

Orcid

-

First Name

Hoda

Last Name

Khalifa

MiddleName

Mahmoud

Affiliation

Histology and cell biology department, Faculty of medicine, Alexandria university, Egypt

Email

hoda.khalifa62@gmail.com

City

Alexandria

Orcid

-

First Name

Silvia

Last Name

Sawires

MiddleName

Kamil

Affiliation

Alexandria Faculty of medicine

Email

silviakamil76@yahoo.com

City

Alexandria

Orcid

-

Volume

46

Article Issue

3

Related Issue

44342

Issue Date

2023-09-01

Receive Date

2022-06-05

Publish Date

2023-09-01

Page Start

1,448

Page End

1,465

Print ISSN

1110-0559

Online ISSN

2090-2417

Link

https://ejh.journals.ekb.eg/article_248606.html

Detail API

https://ejh.journals.ekb.eg/service?article_code=248606

Order

31

Type

Original Article

Type Code

119

Publication Type

Journal

Publication Title

Egyptian Journal of Histology

Publication Link

https://ejh.journals.ekb.eg/

MainTitle

Possible Synergistic Effect of Platelet Rich Plasma and Losartan on Experimentally Induced Skeletal Muscle Injury in Adult Male Albino Rats: A Histological and an Immunohistochem

Details

Type

Article

Created At

23 Dec 2024