This research aimed to examine the cellular response of the juvenile Lithopenaeus vannamei when reared with different levels of technology. The research was carried out by taking samples directly to the locations of semi-intensive ponds and intensive ponds. The frequency of sampling was 2 times at an interval of 15 days when the juvenile Lithopenaeus vannamei were ± 2 months old. The cellular response was observed by monitoring the expression of physiological parameters, namely the hemocyte count and the percentage of hemocyte cell differentiation based on type. The physiological response expressed from the research results showed that there was an increase in the hemocyte count of juvenile Lithopenaeus vannamei during monitoring at 16:30, each increasing from 32×10⁶ cells/ ml in semi-intensive ponds, and 36.8×10⁶ cells/ ml in the intensive ponds. At 12:00 PM monitoring, it was 70.7×10⁶ cells/ ml in the semi-intensive ponds and 76.8×10⁶ cells /ml in intensive ponds. Then, it decreased toward 3:00 AM to 26.8×10⁶ cells/ ml in the intensive ponds and 36.7×10⁶ cells/ ml in the semi-intensive ponds. Furthermore, the percentage of hyaline cells was higher (54- 62%) in the semi-intensive ponds compared to globular cells (31- 35%) and semi-globular cells (6- 65%), but in intensive ponds, globular cells were higher than hyaline cells and globular at every 24-hour monitoring. This fact shows that the vitality condition of the Lithopenaeus vannamei juveniles, based on the cellular response expressed by the animals in the semi-intensive ponds, is better than in the intensive ponds. In intensive ponds, the physiological condition of the Lithopenaus vannamei juveniles is thought to be vulnerable to stress, especially before dawn (4:30 AM).