It is known that there are many problems caused by the use of synthetic hormones on fruit, the most important is that they are not safe for human health in the long term. This work examines some alternatives to synthetic hormones. This research studied the impact of microbial hormones as sources of gibberellins, ascorbic acid, and riboflavin, on the quality of the Thompson grapevine and compared it with synthetic hormones during 2019 and 2020 seasons. The three types of microbial hormones were used alone or combined. synthetic hormones were also used similarly. Some characteristics were measured as indicators of the quality level including yield and the physical and chemical characteristics of berries. The data proved that all treatments, either microbial or synthetic, gave the best effect compared with the control. Microbial hormones had the same effect as synthetic hormones. The best impact was in the combination of the three types of microbial hormones compared with the other treatments.