Faba bean (Vicia fabae) is considered the most important legume crop in Egypt. Eighty fungal isolates were isolated from faba bean leaves cultivated in different localities in Assiut and Sohag governorates. All isolates were identified using morphological characteristics of mycelia and spores' features. These isolates were identified by molecular methods using ITS-rDNA sequencing (ITS1 and ITS4). A resulted based on ITS sequences the fungal samples were Alternaria angustiovoidea (OM432027), Curvularia lunata (OM432028) and Fusarium solani (OM432026). Pathogenicity tests were performed in a greenhouse on faba bean cultivar 843. Different degrees of leaf spot disease symptoms were produced by each tested isolate. A. angustiovoidea C. lunata and F. solani produced the highest disease severity percentage 62.33, 62.66 and 50.23%, respectively. Evaluation of four cultivars reaction to leaf spot disease showed that, Giza 843 was the most susceptible cultivar followed by Giza 125 while, the most tolerant cultivar was Giza 2 followed by Giza 667.