The present study was carried out through two seasons 2018 and 2019 on 26 years old Ruby Seedless grapevines grown at the Experimental Orchard of the Faculty of Agriculture, Assiut University, Egypt, to study the effect of various natural plant extracts; synthetic chemicals and some selected combinations on yield (kg/vine) and cluster weight of Ruby Seedless grape cultivar. The natural plant extracts included Moringa, Fulvic acid, Chitosan, Turmeric extract, Aloe vera and Seaweed while the synthetic chemicals were GA3, Hydrogen peroxide (H2O2), Salicylic acid, Mepiquat chloride, Micronutrients and free amino acids. As well as some combined treatments. The results revealed that most of the treatments significantly increased both yield and cluster weight. The best treatments in this regard were Chitosan extract; Seaweed extract and Fulvic acid while Salicylic acid recorded the best results in this respect. On the other side; free amino acids + Fulvic acid produced the highest yield and cluster weight.Finally, amino acids are basic ingredients in the process of protein synthesis. Using these compounds enhanced yield, fruit quality and vine growth of various grape cultivars.