A field experiment was conducted during 2019/2020 and 2020/2021 seasons to investigate the impact of different concentrations of foliar application of glycine (200 and 400 ppm) and/or Zn (100 and 200 ppm) in addition to the control, and different planting lines (wide and narrow lines) on the growth, yield, chemical content as well as volatile oil and its active components of cumin (Cuminum cyminum L.). The recorded data showed that that wide planting lines (terrace) significantly increased the vegetative (plant height, branch number/plant and fresh & dry weights of herb), yield (number of umbels/plant, weight of thousand seed and fruit yield per plant and per feddan), biochemical constituents (total phenolics, total flavonoids and antioxidant activity) and essential oil parameters (oil percentage, oil yield per plant and per feddan). Spraying cumin plants with glycine (200 and 400 ppm), chelated-zinc (100 and 200 ppm) and their combinations significantly reflected on most recorded parameters. The highest concentration of each substance was superior compared to lower concentration and control. Glycine at 400 ppm plus chelated-Zn at 200 ppm in combination with wide planting lines was the superior treatment and significantly increased all studied parameters compared with other combinations. So, we can recommend applying this treatment to produce clear, safe, and healthy cumin plants.