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347415

Potential Protective Effects of Milk Thistle (Silybum Marianum L.) Seeds Against Benzo[a]Pyrene-Induced Hepatic and Nephritic Injuries in Rats: Biochemical and Histopathological

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Food Science and technology

Abstract

Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, poses a significant threat to human health as an increasingly prevalent environmental and foodborne pollutant. Our previous study revealed that wild Silybum marianum seeds ethanolic extract (SMSE) attenuated B[a]P-induced oxidative stress and cell apoptosis in albino rats. In this work, we extended our hypothesis to investigate the potential protective effects of SMSE against hepatic and nephritic injuries induced by B[a]P. Thirty-six male albino rats were assigned into six groups: Group I, the control (-Ve), received 10 ml/kg/BW corn oil orally twice weekly; Group II, the control (+Ve), was given 50 mg/kg/BW of B[a]P in corn oil twice weekly; and Groups III, V, IV, and VI were orally administered B[a]P + SMSE at 200, 400, 600, and 800 mg/kg BW/day, respectively, for four weeks. B[a]P exposure significantly (p≤0.05) increased the relative weights of the liver (33.33%) and kidney (39.68%); cytochrome P450 (CYP450) activity, a biotransformation marker, (68.25%); tumor necrosis factor-alpha (TNF-α) levels, a pro-inflammatory mediator, (95.95%); liver enzymes activities (AST, 124.09%, ALT, 124.98%, and ALP, 128.77%); and renal function indicators (serum urea, 60.18%, and creatinine, 68.35%), accompanied by severe histopathologic changes in liver and kidney tissues compared to control (-Ve). Conversely, SMSE interventions significantly (p≤0.05) dose-dependently reduced elevated rates of these indices, while improving liver and kidney histoarchitecture. The SMSE's protective effects may result from its bioactive compounds content that regulate B[a]P metabolic enzymes, inhibit CYP450 activity, suppress TNF-α, and scavenge other reactive intermediates. Our results suggest SMSE as a potential natural approach to alleviating environmental pollutants' detrimental effects and deserves further research.
 

DOI

10.21608/asejaiqjsae.2024.347415

Keywords

Silybum marianum, Benzo[a]pyrene, Hepatotoxicity, Nephrotoxicity, Cytochrome P450, inflammatory response, histopathology

Authors

First Name

Yousif Abd El-Aziz

Last Name

Elhassaneen

MiddleName

-

Affiliation

Department of Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt

Email

yousif12@hotmail.com

City

Shebin El-Kom,

Orcid

0000-0003-1391-3653

First Name

Mohamed

Last Name

Mahran

MiddleName

Zakaria

Affiliation

Department of Nutrition and Food Science, Faculty of Home Economics, Menoufia University, Shebin El-Kom, Egypt

Email

mohamed.mahran@hec.menofia.edu.eg

City

Shebin El-Kom

Orcid

0000-0003-2480-8832

Volume

45

Article Issue

1

Related Issue

45054

Issue Date

2024-03-01

Receive Date

2024-02-20

Publish Date

2024-03-30

Page Start

131

Page End

152

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 45_Issue 1_Pages 131-152.pdf

PDF . 1.3MB

Link

https://asejaiqjsae.journals.ekb.eg/article_347415.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=347415

Order

13

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Potential Protective Effects of Milk Thistle (Silybum Marianum L.) Seeds Against Benzo[a]Pyrene-Induced Hepatic and Nephritic Injuries in Rats: Biochemical and Histopathological

Details

Type

Article

Created At

23 Dec 2024