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346752

Modification of Lignin Polymers with COMT Anti-Sense Gene in the Jatropha curcas L. by Green Biotechnology Tools

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Crop genetics and breeding

Abstract

Lignin, a complex aromatic polymer, is considered a part of the secondary cell wall. Also, it's called a "cell adhesive" based on provides strength and rigidity to plant tissues and fibers through strong cross-linking with cell wall components. Moreover, lignin acts as a line of defense for plants against biotic and abiotic stresses. In the field of plant biotechnology, particle bombardment remains the most important technique to directly transfer DNA from source to target organ and the most widely used method to generate transgenic plants for commercial purposes. Down-regulation of Jatropha curcas's lignin-producing caffeic acid 3-O-methyltransferase (COMT) gene production pathway led the transgenic plants to develop cell walls. The transgene COMT antisense is used as a selection marker pZMAS-COMT 10,907 bp plasmid (University of Illinois) to confer resistance to glufosinate herbicides under the 35S promoter. Irradiation of embryonic callus with bullet particles introduced the COMT antisense gene and reduced the amount of lignin. Transgenic green callus was used to extract DNA, RNA, and PCR & RT-PCR were performed to detect the presence of genes. A positive sharp band appeared in the green callus, indicating that the gene expression level was downregulated compared to the control. Transgenic shoots and green callus had lower lignin content compared to controls.
 

DOI

10.21608/asejaiqjsae.2024.346752

Keywords

Jatropha curcas, lignin, Genetic engineering, tissue culture, PCR, particle bombardment

Authors

First Name

Heba

Last Name

Krosh

MiddleName

-

Affiliation

Department of Agricultural Botany, Agriculture Faculty- Saba Basha, Alexandria University.

Email

heba.mahmoud293@yahoo.com

City

-

Orcid

-

First Name

Hossam

Last Name

El-Wakil

MiddleName

-

Affiliation

Department of Agricultural Botany, Agriculture Faculty- Saba Basha, Alexandria University.

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Khaled

MiddleName

-

Affiliation

Department of Agricultural Botany, Agriculture Faculty- Saba Basha, Alexandria University.

Email

-

City

-

Orcid

-

First Name

Manal

Last Name

Abdel-Rahman

MiddleName

-

Affiliation

Genetic Engineering and Biotechnology Laboratory, Department of Plant Pathology, (Genetic Branch), Agriculture Faculty,Damanhour University.

Email

-

City

-

Orcid

-

Volume

45

Article Issue

1

Related Issue

45054

Issue Date

2024-03-01

Receive Date

2024-02-20

Publish Date

2024-03-30

Page Start

95

Page End

106

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 45_Issue 1_Pages 95-106.pdf

PDF . 772.4kB

Link

https://asejaiqjsae.journals.ekb.eg/article_346752.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=346752

Order

10

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Modification of Lignin Polymers with COMT Anti-Sense Gene in the Jatropha curcas L. by Green Biotechnology Tools

Details

Type

Article

Created At

23 Dec 2024