It is known that isoniazid, an anti-mycobacterial medication used clinically to treat bacterial infections (tuberculosis), causes liver damage in both people and animals as well as metabolic malfunction. Antioxidant supplementation, however, may improve the potential adverse effects of anti-tuberculosis drugs. Thus, the purpose of this study is to assess its toxicity in male rat liver cells. Rats weighing between 250 and 300 grams were randomly assigned to three groups, each consisting of six animals: group (1) received water; group (2) received isoniazid medication orally (50 mg/kg); group (3) received vitamin C medication (100 mg/Kg) and isoniazid drug. The levels of plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), and ALP increased significantly (p<0.05) after isoniazid treatment. The toxicity impact caused by the medication was greatly reversed by both the medicine and vitamin C. Histopathological findings indicated a potential protective effects of vitamin C extracts as it prevented isoniazid-induced degenerations. This was in the form of pyknosis, swelling of hepatocyte, foci of necrosis. The findings imply that rats experience hepatotoxicity at a dose of isoniazid. Vitamin C may be used as a protective in tuberculosis therapy and has potential clinical applications in hepatic damage due to its chemo-protective properties during isoniazid treatment.