Bioethanol is a sustainable biofuel generated from lignocellulosic biomass wastes. It has been discovered that adding nutrients and electron acceptors in the form of nanomaterials might alter the bioenvironment and bio-stimulate microorganisms to carry out the intended bioprocesses effectively. In this study, XRD and UV-Vis spectroscopic techniques were used to confirm the purity, structure, and quality of green synthesized ZnO nanoparticles made using crude extract from banana peels. ZnO nanomaterials were used in combination with the control (i.e., no nanomaterials added) to improve the production of bioethanol from sugar cane bagasse. It was proposed that treating yeast with nutrients or nanomaterials would bio-stimulate the cells and boost their activity. It was found that the maximum concentration of bioethanol (19.75 g/L) was produced by bio-stimulating Saccharomyces cerevisiae with 200 mg/L of ZnO nanoparticles, as compared to the control (no nanomaterials added